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PMID: 3001712 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

HLA-B locus polymorphism: studies with a specific hybridization probe.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 24 ·1985-12-00 ·Pages 8614-8

Coppin HL, Denny DW, Weissman SM, McDevitt HO

Abstract

The large number of class I histocompatibility genes (HLA) and their extensive homology has made it difficult to assign bands on genomic Southern blots to known genes. Therefore, we have tried to obtain nucleic acid probes for class I genes that are locus specific or have restricted locus specificity. Computer sequence-homology analysis was used to compare the nucleic acid sequences of two genomic clones, one coding for the HLA-B7 antigen (JY150) and one containing a class I pseudogene (pHLA12.4). A sequence in the 3' untranslated region with very low homology was identified. This sequence from the HLA-B7 gene was subcloned into M13 phage. This fragment, JY150/C5, hybridized with two genomic bands in DNA from human HLA homozygotes--presumably the HLA-B locus gene and a closely related gene. The probe was used to assess restriction fragment polymorphism at the HLA-B locus in homozygous consanguineous cell lines. This analysis permitted the association of certain polymorphic restriction enzyme fragments with some alleles of this locus. However, many HLA-B alleles have identical restriction fragments produced by a number of restriction endonucleases.

MeSH Terms
Alleles Cell Line Chromosome Mapping Cloning, Molecular DNA Restriction Enzymes Genes HLA Antigens/genetics HLA-B Antigens Humans Nucleic Acid Hybridization Polymorphism, Genetic
Chemicals
HLA Antigens HLA-B Antigens DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Coppin H L
Denny D W
Weissman S M
McDevitt H O
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.24.8614
Published
1985-12-00
Pages
8614-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390968
Subset
IM
Grants
NIAID NIH HHS · AI 11313 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.24.8614 PMC Full Text View journal details
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