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PMID: 3004945 Published · ppublish English Journal Article

The abnormal location of cytoplasmic SV40 large T is not caused by failure to bind to DNA or to p53.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3235-40

Paucha E, Kalderon D, Richardson WD, Harvey RW, Smith AE

Abstract

We have examined the large T encoded by an SV40 mutant, d10, which fails to localize to the nucleus. The DNA sequence of the mutant predicts the alteration of Lys 128----Thr within the sequence 127 Lys Lys Lys Arg Lys 131 of large T. The results show that d10 large T is capable of binding to SV40 DNA, to cellular DNA and to the cellular phosphoprotein p53 as well as wild-type large T. These data suggest that the cytoplasmic location of d10 large T is not due to an inability of the protein to be retained within the nucleus, but argues instead that the protein fails to reach the nucleus because it contains a defective nuclear location signal.

MeSH Terms
Adenosine Triphosphate/metabolism Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics,metabolism Cell Line Cell Transformation, Neoplastic DNA Restriction Enzymes Mice Neoplasm Proteins/metabolism Nucleoproteins/metabolism Oncogene Proteins, Viral/genetics,metabolism Phosphoproteins/metabolism Phosphorus Radioisotopes Plasmids Protein Binding Protein Kinases/metabolism Simian virus 40/genetics Tumor Suppressor Protein p53
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Neoplasm Proteins Nucleoproteins Oncogene Proteins, Viral Phosphoproteins Phosphorus Radioisotopes Tumor Suppressor Protein p53 Adenosine Triphosphate Protein Kinases DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Paucha E
Kalderon D
Richardson W D
Harvey R W
Smith A E
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3235-40
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554648
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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