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PMID: 6313352 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Acylated simian virus 40 large T-antigen: a new subclass associated with a detergent-resistant lamina of the plasma membrane.

The EMBO journal ·Vol. 2 ·No. 7 ·1983-00-00 ·Pages 1151-7

Klockmann U, Deppert W

Abstract

We have analyzed the plasma membrane association of the SV40 large tumor antigen (large T) in SV40-transformed BALB/c mouse tumor cells (mKSA). Isolated plasma membranes were subfractionated: treatment with the non-ionic detergent Nonidet P40 (NP40) resulted in a NP40-resistant plasma membrane lamina, which could be further extracted with the zwitterionic detergent Empigen BB. Analysis of the different plasma membrane fractions revealed that only about one third of large T associated with isolated plasma membranes could be solubilized with NP40. The residual plasma membrane-associated large T was tightly bound to the NP40-resistant lamina of the plasma membrane from which it was released by treatment with the zwitterionic detergent Empigen BB. Further evidence for a specific interaction of a distinct subclass of large T with the plasma membrane was provided by showing that only T associated with the NP40-resistant lamina of the plasma membrane contained covalently bound fatty acid. Neither nuclear large T nor large T in the NP40-soluble plasma membrane fraction could be labeled with [3H]palmitic acid. Our results indicate that an acylated subclass of large T interacts specifically with a structure of the plasma membrane, suggesting that it might be involved in a membrane-dependent biological function.

MeSH Terms
Acylation Animals Antigens, Viral, Tumor/classification Cell Line Cell Membrane/immunology Cell Transformation, Viral Detergents/pharmacology Mice Simian virus 40/immunology
Chemicals
Antigens, Viral, Tumor Detergents
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Klockmann U
Deppert W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1983-00-00
Pages
1151-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC555249
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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