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PMID: 3010228 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing in the external transcribed spacer of ribosomal RNA from Physarum polycephalum.

Nucleic acids research ·Vol. 14 ·No. 8 ·1986-04-25 ·Pages 3153-66

Blum B, Pierron G, Seebeck T, Braun R

Abstract

The rDNA of the myxomycete Physarum polycephalum is transcribed to give a 13.3 kb precursor of ribosomal RNA. At 1.7 kb downstream of the primary initiation site there is a processing site or a second initiation site. This site was studied by S1-mapping, DNA sequencing and electron microscopy. None of these methods could conclusively distinguish between the two formal possibilities. However, capping experiments indicate that rapid processing is taking place at this site rather than reinitiation. In addition, primary transcripts and processed molecules were assayed throughout the synchronous mitotic cycle. During all interphase stages newly initiated transcripts of rDNA and products of the first processing step are present in similar amounts, indicating control of initiation and not of maturation as being the main regulatory step for the accumulation of mature rRNAs. During the brief period of mitosis the level of newly initiated rRNA precursors is lowered.

MeSH Terms
Base Sequence Chromatin/ultrastructure DNA Restriction Enzymes Endonucleases Microscopy, Electron Nucleic Acid Precursors/genetics Physarum/genetics Plasmids RNA Caps/genetics RNA Precursors RNA Processing, Post-Transcriptional RNA, Ribosomal/genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Chromatin Nucleic Acid Precursors RNA Caps RNA Precursors RNA, Ribosomal Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blum B
Pierron G
Seebeck T
Braun R
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.8.3153
Published
1986-04-25
Pages
3153-66
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339739
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/14.8.3153 PMC Full Text View journal details
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