Membrane permeability changes associated with DNA gyrase inhibitors in Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 28 ·No. 2 ·1985-08-00 ·Pages 200-6

Dougherty TJ, Saukkonen JJ

Abstract

Inhibition of DNA synthesis in Escherichia coli B/r by a DNA gyrase inhibitor results in cell death after a 50-min lag period. Examination of the cells under phase-contrast and electron microscopes revealed that they appeared to undergo plasmolysis coincident with the onset of cell death. The inhibited cells were also found to become susceptible to low levels of detergent at this time. With a fluorescent membrane probe, the level of membrane permeability was assessed and found to increase concurrently with the decrease in culture viability. Analysis of the cell envelope constituents revealed that, other than a shift in the protein/lipid ratio, the compositions of the cell membranes were unperturbed.

MeSH Terms

Bacterial Proteins/analysis Cell Membrane Permeability/drug effects Escherichia coli/analysis,drug effects Membrane Lipids/analysis Membrane Proteins/analysis Molecular Weight Nalidixic Acid/pharmacology Sodium Dodecyl Sulfate/pharmacology Topoisomerase II Inhibitors

Chemicals

Bacterial Proteins Membrane Lipids Membrane Proteins Topoisomerase II Inhibitors Sodium Dodecyl Sulfate Nalidixic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dougherty T J

Saukkonen J J

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25 references, click to expand

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Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.28.2.200

Published

1985-08-00

Pages

200-6

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC180219

Subset

External Links

PubMed source DOI: 10.1128/AAC.28.2.200 PMC Full Text View journal details

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Membrane permeability changes associated with DNA gyrase inhibitors in Escherichia coli.

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