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PMID: 6324197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 5 ·1984-03-00 ·Pages 1499-503

Krueger JH, Walker GC

Abstract

Two proteins with molecular weights of 61,000 and 73,000 were found to be induced by UV light in Escherichia coli mutants in which the SOS responses are constitutively expressed. The induction of these proteins by UV light and nalidixic acid was shown to be independent of the recA+ lexA+ regulatory system. Analysis of these proteins by two-dimensional gel electrophoresis and comparison with the "heat-shock" proteins of E. coli revealed that the Mr 61,000 protein comigrated with the groEL gene product, that the Mr 73,000 protein comigrated with the dnaK gene product, and that other heat-shock proteins were also induced. The induction of groEL and dnaK by UV light and nalidixic acid is controlled by the htpR locus. The results suggest that the regulatory response of E. coli to agents such as UV light and nalidixic acid is more complex than previously thought.

MeSH Terms
Alleles Bacterial Proteins/genetics,isolation & purification DNA Repair Escherichia coli/drug effects,genetics,radiation effects Genes/drug effects,radiation effects Genes, Bacterial/drug effects,radiation effects Molecular Weight Nalidixic Acid/pharmacology Species Specificity Ultraviolet Rays
Chemicals
Bacterial Proteins Nalidixic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Krueger J H
Walker G C
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.5.1499
Published
1984-03-00
Pages
1499-503
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344864
Subset
IM
Grants
NIGMS NIH HHS · GM28988 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.5.1499 PMC Full Text View journal details
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