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PMID: 6821127 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SOS chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5971-5

Quillardet P, Huisman O, D'Ari R, Hofnung M

Abstract

We present and evaluate the SOS chromotest, a bacterial test for detecting DNA-damaging agents. It is a colorimetric assay based on the induction by these agents of the SOS function sfiA, whose level of expression is monitored by means of a sfiA::lacZ operon fusion. The response is rapid (a few hours), and does not require survival of the tester strain. Dose-response curves for various chemicals include a linear region. The slope of this region is taken as a measure of the SOS inducing potency. Comparison for a number of substances of known genotoxicity of the SOS inducing potency determined in the SOS chromotest with the mutagenic potency determined in the Salmonella assay (mutatest) revealed a striking quantitative correlation over more than 7 orders of magnitude. The sensitivity of the SOS chromotest (lowest amount detected) is equal to that of the mutatest and generally 4-40 times higher than that of a phage induction assay (inductest). From a practical point of view our observations contribute to the validation of the SOS chromotest as a test for detecting genotoxins and in particular genotoxic carcinogens. From a theoretical standpoint the results suggest that mutagenic potency measured in the mutatest reflects the level of induction of an SOS function and that most genotoxins are inducers of the SOS response in bacteria.

MeSH Terms
Alkaline Phosphatase/genetics Carcinogens/toxicity Colorimetry/methods DNA Repair Escherichia coli/drug effects,enzymology,genetics Mutagenicity Tests Mutagens Mutation Operon beta-Galactosidase/genetics
Chemicals
Carcinogens Mutagens Alkaline Phosphatase beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Quillardet P
Huisman O
D'Ari R
Hofnung M
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.19.5971
Published
1982-10-00
Pages
5971-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347033
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.19.5971 PMC Full Text View journal details
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