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PMID: 3012122 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (Dearing).

Journal of virology ·Vol. 59 ·No. 1 ·1986-07-00 ·Pages 90-7

Kaye KM, Spriggs DR, Bassel-Duby R, Fields BN, Tyler KL

Abstract

In this paper we provide a step by step comparison of the pathogenesis of murine infection caused by reovirus type 3 (Dearing) and an antigenic variant (K) selected by its resistance to neutralization with a monoclonal antibody (G5) directed against the T3 hemagglutinin. To show that specific changes in the biologic properties of variant K were due to mutation in the S1 double-stranded RNA segment (gene), which encodes the viral hemagglutinin, we generated a reassortant virus ("1 HA K") containing the variant K S1 gene and compared its properties to variant K and to a reassortant ("1 HA 3") containing the T3 (Dearing) S1 gene. These studies, in conjunction with our previous nucleotide sequence analysis of the S1 genes of variant K and T3 (Dearing) [R. Bassel-Duby, A. Jayasuriya, D. Chatterjee, N. Sonenberg, J. V. Maizel, Jr., and B. N. Fields, Nature (London) 315:421-423, 1985; R. Bassel-Duby, D. R. Spriggs, K. L. Tyler, and B. N. Fields, submitted for publication], indicate that a single amino acid change in the T3 hemagglutinin can alter viral growth and tropism within the central nervous system without affecting either its primary replication in the intestine or its pattern of spread to or within the central nervous system.

MeSH Terms
Animals Brain/microbiology Genes, Viral Hemagglutinins, Viral/genetics,immunology Intestines/microbiology Mammalian orthoreovirus 3/genetics,growth & development,immunology,pathogenicity Mice Neutralization Tests Reoviridae/genetics Reoviridae Infections/microbiology Spleen/microbiology Time Factors Viral Proteins/genetics,immunology Virus Replication
Chemicals
Hemagglutinins, Viral Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kaye K M
Spriggs D R
Bassel-Duby R
Fields B N
Tyler K L
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.59.1.90-97.1986
Published
1986-07-00
Pages
90-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253042
Subset
IM
Grants
NIAID NIH HHS · 5 K11 AI00610-02 · United States
NINDS NIH HHS · 5 P50 NS16998-05 · United States
External Links
PubMed source DOI: 10.1128/JVI.59.1.90-97.1986 PMC Full Text View journal details
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