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PMID: 3012274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Shadow promoters in the F plasmid transfer operon.

Molecular & general genetics : MGG ·Vol. 202 ·No. 3 ·1986-03-00 ·Pages 509-11

Fowler T, Thompson R

Abstract

The in vivo transcription start site for PYZ, the promoter of the F plasmid transfer operon, has been located. When transcription start sites for PYZ are deleted, two additional shadow promoters, upstream of PYZ, are activated in vivo. The shadow promoters correspond to those previously identified by in vitro run-off transcription experiments. The activated shadow promoters are stimulated by TraJ protein, a positive regulator of the transfer operon.

MeSH Terms

Base Sequence Chromosome Deletion Chromosome Mapping DNA Restriction Enzymes Endonucleases Escherichia coli/genetics F Factor Operon Promoter Regions, Genetic Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic

Chemicals

Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fowler T

Thompson R

References (11)

11 references, click to expand

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J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.
Mol Gen Genet. 1982;188(3):513-8 PMID: 6298579
Promoters of the malEFG and malK-lamB operons in Escherichia coli K12.
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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00333285

Published

1986-03-00

Pages

509-11

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00333285

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Shadow promoters in the F plasmid transfer operon.

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