Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.

Molecular & general genetics : MGG ·Vol. 188 ·No. 3 ·1982-00-00 ·Pages 513-8

Thompson R, Taylor L

Abstract

The nucleotide sequence of the DNA encoding the traM, finP and the promoter proximal segment of the traJ gene of the F plasmid has been determined. The predicted amino acid sequence for the traM protein shows that this inner membrane protein contains no signal sequence. The promoters for both the traM and traJ genes have been mapped by in vitro transcription and nuclease S1 protection experiments. No unambiguous location can be assigned to the finP gene but all candidates, if translated, would encode small proteins of between 24 and 52 amino acids.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chromosome Deletion DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Operon Plasmids Transcription, Genetic

Chemicals

Bacterial Proteins DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Thompson R

Taylor L

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32 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00330058

Published

1982-00-00

Pages

513-8

Language

English

Region

Germany

NLM ID

0125036

Subset

Databases

GENBANK

K00381, K01147

External Links

PubMed source DOI: 10.1007/BF00330058

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Promoter mapping and DNA sequencing of the F plasmid transfer genes traM and traJ.

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