Home LiteratureArticle Details
PMID: 3016480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VII. RNA polymerase is a target for ppGpp.

Molecular & general genetics : MGG ·Vol. 203 ·No. 2 ·1986-05-00 ·Pages 265-8

Glass RE, Jones ST, Ishihama A

Abstract

RNA polymerase was isolated from two suppressed rpoB amber strains exhibiting relaxed control over RNA synthesis in vivo. In vitro transcription analysis of these mutant holoenzymes carrying defined substitutions at known sites in the beta subunit clearly shows that single amino acid changes in beta render RNA polymerase resistant to ppGpp. This unambiguously demonstrates that RNA polymerase is indeed the target for ppGpp.

MeSH Terms
DNA Restriction Enzymes DNA-Directed RNA Polymerases/genetics,isolation & purification,metabolism Escherichia coli/enzymology,genetics Genotype Guanine Nucleotides/metabolism Guanosine Tetraphosphate/metabolism Templates, Genetic Transcription, Genetic
Chemicals
Guanine Nucleotides Guanosine Tetraphosphate DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Glass R E
Jones S T
Ishihama A
References (21)
21 references, click to expand
  1. Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VI. A redundant region in the beta polypeptide.
    Mol Gen Genet. 1984;196(1):64-7 PMID: 6384732
  2. Genetic studies on the beta subunit of Escherichia coli RNA polymerase. I. The effect of known, single amino acid substitutions in an essential protein.
    Mol Gen Genet. 1982;188(3):399-404 PMID: 6761548
  3. Heterogeneity of RNA polymerase in Escherichia coli. I. A new holoenzyme containing a new sigma factor.
    J Mol Biol. 1974 Mar;83(3):353-67 PMID: 4207198
  4. Control of messenger RNA synthesis and decay in Escherichia coli.
    J Mol Biol. 1966 Oct;20(3):559-73 PMID: 5338988
  5. Determination of the promoter strength in the mixed transcription system. II. Promoters of ribosomal RNA, ribosomal protein S1 and recA protein operons from Escherichia coli.
    Nucleic Acids Res. 1983 Jun 25;11(12):3873-88 PMID: 6346267
  6. The mechanism of action of ppGpp on rRNA synthesis in vitro.
    Cell. 1976 May;8(1):123-8 PMID: 8211
  7. Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.
    Nat New Biol. 1973 May 2;243(122):13-5 PMID: 17319071
  8. Promoter selectivity of Escherichia coli RNA polymerase. Differential stringent control of the multiple promoters from ribosomal RNA and protein operons.
    J Biol Chem. 1984 Feb 10;259(3):1951-7 PMID: 6363418
  9. Regulation of membrane phospholipid synthesis by the relA gene: dependence on ppGpp levels.
    Biochemistry. 1976 Jun 15;15(12):2546-50 PMID: 779827
  10. Genetic studies on the beta subunit of Escherichia coli RNA polymerase. III. Analysis of low-level rifampicin-resistant mutants.
    Mol Gen Genet. 1983;190(3):523-6 PMID: 6348484
  11. A simple procedure for resolution of Escherichia coli RNA polymerase holoenzyme from core polymerase.
    Arch Biochem Biophys. 1977 Aug;182(2):404-8 PMID: 332084
  12. Effects of guanosine tetraphosphate, guanosine pentaphosphate, and beta-gamma methylenyl-guanosine pentaphosphate on gene expression of Escherichia coli in vitro.
    Proc Natl Acad Sci U S A. 1974 Jan;71(1):63-7 PMID: 4359331
  13. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  14. Determination of the promoter strength in the mixed transcription system: promoters of lactose, tryptophan and ribosomal protein L10 operons from Escherichia coli.
    Nucleic Acids Res. 1983 Feb 11;11(3):671-86 PMID: 6300761
  15. The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.
    Eur J Biochem. 1981 Jun 1;116(3):621-9 PMID: 6266829
  16. Modulation of RNA polymerase specificity by ppGpp.
    Mol Gen Genet. 1976 Aug 19;147(2):225-32 PMID: 787760
  17. Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase.
    Biochemistry. 1979 Apr 3;18(7):1344-52 PMID: 371677
  18. Control of rRNA and tRNA syntheses in Escherichia coli by guanosine tetraphosphate.
    J Bacteriol. 1982 Sep;151(3):1261-8 PMID: 6179924
  19. Stringent control in E. coli.
    Annu Rev Genet. 1979;13:393-415 PMID: 94252
  20. Relaxed mutants of Escherichia coli RNA polymerase.
    FEBS Lett. 1983 Mar 21;153(2):307-10 PMID: 6352328
  21. Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1564-8 PMID: 4576025
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00333964
Published
1986-05-00
Pages
265-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00333964
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2