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PMID: 3016512 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.

Molecular and cellular biology ·Vol. 5 ·No. 10 ·1985-10-00 ·Pages 2713-9

Sadofsky M, Connelly S, Manley JL, Alwine JC

Abstract

Our previous studies of the 3'-end processing of simian virus 40 late mRNAs indicated the existence of an essential element (or elements) downstream of the AAUAAA signal. We report here the use of transient expression analysis to study a functional element which we located within the sequence AGGUUUUUU, beginning 59 nucleotides downstream of the recognized signal AAUAAA. Deletion of this element resulted in (i) at least a 75% drop in 3'-end processing at the normal site and (ii) appearance of readthrough transcripts with alternate 3' ends. Some flexibility in the downstream position of this element relative to the AAUAAA was noted by deletion analysis. Using computer sequence comparison, we located homologous regions within downstream sequences of other genes, suggesting a generalized sequence element. In addition, specific complementarity is noted between the downstream element and U4 RNA. The possibility that this complementarity could participate in 3'-end site selection is discussed.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Viral/genetics Gene Expression Regulation Genetic Linkage Nucleic Acid Hybridization Plasmids RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA, Small Nuclear/genetics Sequence Homology, Nucleic Acid Simian virus 40/genetics
Chemicals
DNA, Viral RNA, Messenger RNA, Small Nuclear
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sadofsky M
Connelly S
Manley J L
Alwine J C
References (43)
43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.10.2713-2719.1985
Published
1985-10-00
Pages
2713-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367009
Subset
IM
Grants
NCI NIH HHS · CA28379 · United States
NIGMS NIH HHS · GM 28983 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.10.2713-2719.1985 PMC Full Text View journal details
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