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PMID: 3017841 Published · ppublish English Journal Article

A routine method for the establishment of permanent growing lymphoblastoid cell lines.

Human genetics ·Vol. 73 ·No. 4 ·1986-08-00 ·Pages 320-6

Neitzel H

Abstract

Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Epstein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.

MeSH Terms
Antigens, Viral/analysis B-Lymphocytes/cytology,microbiology,ultrastructure Cell Division/drug effects Cell Line Cell Transformation, Viral Chromosome Aberrations Cyclosporins/pharmacology Epstein-Barr Virus Nuclear Antigens Ethidium/pharmacology Herpesvirus 4, Human/immunology Humans Karyotyping
Chemicals
Antigens, Viral Cyclosporins Epstein-Barr Virus Nuclear Antigens Ethidium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Neitzel H
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51 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00279094
Published
1986-08-00
Pages
320-6
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00279094 View journal details
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