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PMID: 3018430 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterologous expression and regulation of the lysA genes of Pseudomonas aeruginosa and Escherichia coli.

Molecular & general genetics : MGG ·Vol. 203 ·No. 3 ·1986-06-00 ·Pages 430-4

Martin C, Cami B, Borne F, Jeenes DJ, Haas D, Patte JC

Abstract

The Pseudomonas aeruginosa lysA gene encoding diaminopimelate decarboxylase (DAP-decarboxylase) was cloned into a broad host range vector. This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli, where the Pseudomonas lysA gene was poorly expressed. By contrast, the E. coli lysA gene was expressed well in P. aeruginosa and subject to lysine regulation when the E. coli LysR activator protein was provided. This indicates that the mechanism of transcriptional activation for the E. coli lysA gene is effective in the heterologous host.

MeSH Terms
Bacterial Proteins Carboxy-Lyases/genetics Cloning, Molecular Conjugation, Genetic DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Genetic Vectors Genotype Plasmids Pseudomonas aeruginosa/enzymology,genetics
Chemicals
Bacterial Proteins DNA Restriction Enzymes Carboxy-Lyases LysA protein, Bacteria diaminopimelic acid decarboxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Martin C
Cami B
Borne F
Jeenes D J
Haas D
Patte J C
References (28)
28 references, click to expand
  1. Expression of biosynthetic genes from Pseudomonas aeruginosa and Escherichia coli in the heterologous host.
    Mol Gen Genet. 1986 Jun;203(3):421-9 PMID: 3018429
  2. Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. I. Identification of a lysR gene encoding an activator of the lysA gene.
    J Mol Biol. 1983 Aug 5;168(2):307-20 PMID: 6411928
  3. Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication.
    Gene. 1982 Jan;17(1):79-89 PMID: 6281139
  4. Genetic recombination in Pseudomonas aeruginosa.
    J Gen Microbiol. 1955 Dec;13(3):572-81 PMID: 13278508
  5. [Lysine biosynthesis of Pseudomonas aeruginosa PAO1. III. Further characterization of lysine auxotrophic mutant of Ps. aeruginosa PAO1].
    Z Allg Mikrobiol. 1981;21(4):343-6 PMID: 6794230
  6. Construction of hybrid plasmids containing the lysA gene of Escherichia coli: studies of expression in Escherichia coli and Saccharomyces cerevisiae.
    Mol Gen Genet. 1981;182(3):456-61 PMID: 6272062
  7. Identification of the AraE transport protein of Escherichia coli.
    Biochem J. 1981 Apr 15;196(1):269-83 PMID: 7030324
  8. Molecular cloning of TOL genes xylB and xylE in Escherichia coli.
    J Bacteriol. 1981 Mar;145(3):1137-43 PMID: 7009570
  9. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
  10. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  11. The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.
    Mol Gen Genet. 1977 Jul 7;154(1):7-22 PMID: 408599
  12. Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled-expression vectors.
    Gene. 1983 Dec;26(2-3):273-82 PMID: 6323265
  13. Regulatory pattern of the Escherichia coli lysA gene: expression of chromosomal lysA-lacZ fusions.
    J Bacteriol. 1983 Dec;156(3):1198-203 PMID: 6417111
  14. Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. III. Nucleotide sequence and regulation of the lysR gene.
    J Mol Biol. 1983 Aug 5;168(2):333-50 PMID: 6350602
  15. Escherichia coli and Pseudomonas putida RNA polymerases display identical contacts with promoters.
    Mol Gen Genet. 1984;195(3):511-5 PMID: 6236350
  16. Multiple sequences related to classical histocompatibility antigens in the mouse genome.
    Nature. 1981 Jun 25;291(5817):673-5 PMID: 6264319
  17. A mutant sex factor of Pseudomonas aeruginosa.
    Genet Res. 1972 Feb;19(1):91-108 PMID: 4623516
  18. Genetic and molecular characterization of the Pseudomonas plasmid pVS1.
    Plasmid. 1984 May;11(3):206-20 PMID: 6087391
  19. Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2.
    J Mol Appl Genet. 1985;3(1):26-35 PMID: 3839252
  20. Molecular cloning of the Pseudomonas carboxypeptidase G2 gene and its expression in Escherichia coli and Pseudomonas putida.
    J Bacteriol. 1983 Dec;156(3):1222-7 PMID: 6358192
  21. Transcription of the TOL plasmid toluate catabolic pathway operon of Pseudomonas putida is determined by a pair of co-ordinately and positively regulated overlapping promoters.
    EMBO J. 1984 Nov;3(11):2461-6 PMID: 6096122
  22. The relA locus and the regulation of lysine biosynthesis in Escherichia coli.
    Mol Gen Genet. 1980;179(2):319-25 PMID: 6110161
  23. Expression of the argF gene of Pseudomonas aeruginosa in Pseudomonas aeruginosa, Pseudomonas putida, and Escherichia coli.
    J Bacteriol. 1983 Apr;154(1):508-12 PMID: 6403512
  24. Chromosomal genetics of Pseudomonas.
    Microbiol Rev. 1979 Mar;43(1):73-102 PMID: 111024
  25. Role of lysyl-tRNA in the regulation of lysine biosynthesis in Escherichia coli K12.
    Biochimie. 1976;58(1-2):213-8 PMID: 8152
  26. Transposition of TnA does not generate deletions.
    Mol Gen Genet. 1977 Jul 20;154(2):205-11 PMID: 331086
  27. Alterations in host specificity of bacterial deoxyribonucleic acid after an increase in growth temperature of Pseudomonas aeruginosa.
    J Bacteriol. 1966 Jul;92(1):43-8 PMID: 4957436
  28. Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. II. Nucleotide sequence of the lysA gene and its regulatory region.
    J Mol Biol. 1983 Aug 5;168(2):321-31 PMID: 6350601
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00422067
Published
1986-06-00
Pages
430-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00422067
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