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PMID: 6236350 Published · ppublish English Journal Article

Escherichia coli and Pseudomonas putida RNA polymerases display identical contacts with promoters.

Molecular & general genetics : MGG ·Vol. 195 ·No. 3 ·1984-00-00 ·Pages 511-5

Gragerov AI, Chenchik AA, Aivasashvilli VA, Beabealashvilli RSh, Nikiforov VG

Abstract

Methylation protection experiments with four promoters (P1 and P2 of the pBR322 plasmid, lacUV5 and lambda P0) have shown that the RNA polymerases from Escherichia coli and Pseudomonas putida, while differing in the primary structure of the subunits involved in DNA binding, display identical patterns of DNA contacts. Nor do these enzymes differ in covalent cross-linking patterns with a partially apurinized promoter. We conclude that the two RNA polymerases have very similar structures of DNA binding centers. The evolutionary conservation of this structure may account for the fact that diverse RNA polymerases often recognize and efficiently use promoters of distant bacterial species.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence DNA, Bacterial/metabolism DNA, Viral/genetics DNA-Directed RNA Polymerases/genetics Escherichia coli/enzymology,genetics,metabolism Methylation Operon Pseudomonas/enzymology,genetics,metabolism Purines/metabolism Transcription, Genetic
Chemicals
DNA, Bacterial DNA, Viral Purines DNA-Directed RNA Polymerases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gragerov A I
Chenchik A A
Aivasashvilli V A
Beabealashvilli R Sh
Nikiforov V G
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38 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00341455
Published
1984-00-00
Pages
511-5
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00341455
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