Promoter sites in the genome of B. subtilis phage SPP1.
Transcriptional complexes formed in vitro using DNA of B. subtilis phage SPP1 as template and E. coli and B. subtilis RNA polymerases were analyzed by electron microscopy. Both enzymes recognize the same five strong promoters in the early region of the genome. Strand selection at these sites was identical with both enzymes. These results correlate well with data obtained from in vivo transcription studies. Transcriptional activity in the late region of the genome was very low, not permitting the identification of promoter sites.
19 references, click to expand
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Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III.Mol Gen Genet. 1976 Dec 8;149(2):131-4 PMID: 827685
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The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.Mol Gen Genet. 1979 Jan 10;168(2):165-72 PMID: 109739
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Asymmetric transcription of SPP1 in vivo.Mol Gen Genet. 1973 Jul 31;124(1):57-63 PMID: 4200375
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Asymmetric transcription of B. subtilis phage SPP1 DNA in vitro.Biochem Biophys Res Commun. 1969 Mar 31;34(6):824-30 PMID: 4888715
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Transcription after bacteriophage SPP1 infection in Bacillus subtilis.J Virol. 1972 Aug;10(2):187-92 PMID: 4627475
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Length measurements of RNA synthesized in vitro by Escherichia coli RNA polymerase.J Mol Biol. 1973 Mar 15;74(4):677-87 PMID: 4354077
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The genome of B. subtilis phage SSP1: the topology of DNA molecules.Mol Gen Genet. 1979 Jan 10;168(2):153-61 PMID: 109737
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Purification and properties of DNA-dependent RNA polymerase from Bacillus subtilis vegetative cells.Eur J Biochem. 1971 Aug 25;21(4):526-35 PMID: 4999759
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A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
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In vivo transcription of Bacillus subtilis bacteriophage SPP1.Mol Gen Genet. 1981;181(4):512-7 PMID: 6267424
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Gene expression of bacteriophage SPP1. II. Regulatory aspects.Mol Gen Genet. 1975 Dec 23;142(1):57-66 PMID: 814402
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Interaction of E. coli RNA polymerase with promotors of coliphage T5: the rates of complex formation and decay and their correlation with in vitro and in vivo transcriptional activity.Mol Gen Genet. 1977 Dec 9;157(3):301-11 PMID: 340927
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Gene expression of bacteriophage SPPI. I. Phage directed protein synthesis.Mol Gen Genet. 1975 Dec 23;142(1):45-55 PMID: 814401
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Organization of transcriptional signals in plasmids pBR322 and pACYC184.Proc Natl Acad Sci U S A. 1981 Jan;78(1):167-71 PMID: 7017708
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The genome of B. subtilis phage SPP1: physical arrangement in phage genes.Mol Gen Genet. 1979 Oct 1;175(3):351-7 PMID: 118320
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Electron microscopic analysis of in vitro transcriptional complexes: mapping of promoters of the coliphage T5 genome.Mol Gen Genet. 1978 Oct 30;166(2):141-9 PMID: 745595
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Cloning and expression of Bacillus subtilis phage SPP1 in E. coli. II. Expression of lambda/SPP1 hybrid phages in E. coli minicells.Mol Gen Genet. 1981;182(2):299-303 PMID: 6457236
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The genome of B. subtilis phage SPP1: assignment of 5'--3'-polarity to the complementary strands of SPP1 DNA.Mol Gen Genet. 1978 Aug 4;164(1):93-7 PMID: 100673
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Restriction fragment analysis of bacteriophage SPP1 in vitro transcription by host RNA polymerase.J Virol. 1978 Oct;28(1):95-105 PMID: 100614
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