Cloning and expression of Bacillus subtilis phage SPP1 in E. coli. II. Expression of lambda/SPP1 hybrid phages in E. coli minicells.
In the preceding paper (Amann et al. 1981) we described the in vitro construction of hybrids between Escherichia coli phage lambda NM607 imm434 and B. subtilis phage SPP1. These lambda/SPP1 hybrids have been used to infect minicells produced by E. coli strain DS410. Analysis on polyacrylamide gels of 35S-methionine labeled proteins synthesized in infected minicells revealed the expression of both lambda and SPP1 genes. Infection of E. coli minicells carrying plasmid pGY101, which encodes and expresses the repressor gene of phage 434, results in the selective expression of the cloned SPP1 DNA. This has resulted in the assignment of 26 out of a total of 46 known SPP1 polypeptides (Mertens et al. 1979) to individual SPP1 DNA fragments. In addition, several lambda/SPP1 fusion peptides whose transcription either originates from lambda promoters or from promoters located on the inserted SPP1 fragment, were identified.
29 references, click to expand
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Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III.Mol Gen Genet. 1976 Dec 8;149(2):131-4 PMID: 827685
-
Cellular levels of the prophage lambda and 434 repressors.J Mol Biol. 1979 Jul 5;131(3):655-61 PMID: 159957
-
Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.Mol Gen Genet. 1978 Nov 9;166(3):277-85 PMID: 105242
-
The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.Mol Gen Genet. 1979 Jan 10;168(2):165-72 PMID: 109739
-
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
-
Lambda encodes an outer membrane protein: the lom gene.Mol Gen Genet. 1979;172(3):243-8 PMID: 45607
-
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
-
Cloning and expression of the Bacillus subtilis phage SPP1 in E. coli. I. Construction and characterization of lambda/SPP1 hybrids.Mol Gen Genet. 1981;182(2):293-8 PMID: 6457235
-
DNA of Bacillus subtilis bacteriophage SPP1: physical mapping and localization of the origin of replication.J Virol. 1978 Dec;28(3):865-76 PMID: 104053
-
Transcription after bacteriophage SPP1 infection in Bacillus subtilis.J Virol. 1972 Aug;10(2):187-92 PMID: 4627475
-
The genome of B. subtilis phage SSP1: the topology of DNA molecules.Mol Gen Genet. 1979 Jan 10;168(2):153-61 PMID: 109737
-
Bacteriophage infection of minicells: a general method for identification of "in vivo" bacteriophage directed polypeptide biosynthesis.Mol Gen Genet. 1977 Dec 14;158(1):73-9 PMID: 415222
-
Intermediate steps in the degradation of a specific abnormal protein in Escherichia coli.J Biol Chem. 1977 Dec 10;252(23):8350-7 PMID: 336618
-
DNA- and RNA-directed synthesis in vitro of phage enzymes.Methods Enzymol. 1974;30:654-68 PMID: 4604361
-
Use of minicells for bacteriophage-directed polypeptide synthesis.Methods Enzymol. 1979;68:493-503 PMID: 542128
-
Gene expression of bacteriophage SPP1. II. Regulatory aspects.Mol Gen Genet. 1975 Dec 23;142(1):57-66 PMID: 814402
-
Lambdoid phages that simplify the recovery of in vitro recombinants.Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
-
Synthesis of cell envelope components by anucleate cells (minicells) of Bacillus subtilis.J Bacteriol. 1977 Mar;129(3):1198-207 PMID: 403171
-
Gene expression of bacteriophage SPPI. I. Phage directed protein synthesis.Mol Gen Genet. 1975 Dec 23;142(1):45-55 PMID: 814401
-
Mutants of Escherichia coli with a defect in the degradation of nonsense fragments.Nat New Biol. 1973 Jun 20;243(129):238-41 PMID: 4577438
-
Bacteriophage SPP1 polypeptides synthesized in infected minicells and in vitro.Mol Gen Genet. 1979;172(3):271-9 PMID: 45610
-
Inactivation of prophage lambda repressor in vivo.J Mol Biol. 1979 Jul 5;131(3):553-72 PMID: 159955
-
Protein expression in E. coli minicells by recombinant plasmids.Cell. 1977 Mar;10(3):521-36 PMID: 403011
-
Bacillus subtilis DNA polymerase III is required for the replication of DNA of bacteriophages SPP-1 and phi 105.J Virol. 1977 Feb;21(2):493-6 PMID: 401898
-
Minicells of Bacillus subtilis.J Bacteriol. 1973 May;114(2):860-73 PMID: 4196259
-
Selective expression of transduced or cloned DNA in minicells containing plasmid pKB280.Nature. 1978 Dec 14;276(5689):728-9 PMID: 366435
-
The genome of B. subtilis phage SPP1: physical arrangement in phage genes.Mol Gen Genet. 1979 Oct 1;175(3):351-7 PMID: 118320
-
Analysis of bacteriophage T7 early RNAs and proteins on slab gels.J Mol Biol. 1973 Sep 15;79(2):237-48 PMID: 4760132
-
The genome of B. subtilis phage SPP1: assignment of 5'--3'-polarity to the complementary strands of SPP1 DNA.Mol Gen Genet. 1978 Aug 4;164(1):93-7 PMID: 100673
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