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PMID: 45610 Published · ppublish English Comparative Study Journal Article

Bacteriophage SPP1 polypeptides synthesized in infected minicells and in vitro.

Molecular & general genetics : MGG ·Vol. 172 ·No. 3 ·1979-00-00 ·Pages 271-9

Mertens G, Amann E, Reeve JN

Abstract

Minicells produced by B. subtilis CU403divIVB1 and infected by SPP1 synthesize at least 46 polypeptides which can be separated by polyacrylamide gel electrophoresis. These polypeptides represent the expression of 86% of the SPP1 genome's coding capacity. Infection of minicells by sus mutants and deletion mutants of SPP1 has permitted a correlation of genetic location with gene product and has shown that SPP1 normally synthesizes at least 8 non-essential polypeptides. Restriction fragments of SPP1 produced by EcoRI digestion of SPP1 DNA have been purified and used as template DNA in a coupled transcription/translation system derived from E. coli to determine the polypeptides encoded by the individual fragments. SPP1 expression in minicells differs from SPP1 expression in nucleated cells (Esche, 1975) in that late syntheses are not dependent on phage DNA replication in infected minicells.

MeSH Terms
Bacillus subtilis/metabolism Bacteriophages/genetics,metabolism Capsid/genetics,metabolism Cell-Free System DNA, Viral/genetics Escherichia coli Gene Expression Regulation, Viral Genes, Viral Mutation Peptide Biosynthesis Peptides/genetics Viral Proteins/biosynthesis,genetics Viral Structural Proteins/genetics
Chemicals
DNA, Viral Peptides Viral Proteins Viral Structural Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mertens G
Max-Planck-Institut für molekulare Genetik, Abt. Trautner, Berlin.
Amann E
Reeve J N
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20 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271726
Published
1979-00-00
Pages
271-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271726
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