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PMID: 3018499 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A single N-linked oligosaccharide at either of the two normal sites is sufficient for transport of vesicular stomatitis virus G protein to the cell surface.

Molecular and cellular biology ·Vol. 5 ·No. 11 ·1985-11-00 ·Pages 3074-83

Machamer CE, Florkiewicz RZ, Rose JK

Abstract

We investigated the role of glycosylation in intracellular transport and cell surface expression of the vesicular stomatitis virus glycoprotein (G) in cells expressing G protein from cloned cDNA. The individual contributions of the two asparagine-linked glycans of G protein to cell surface expression were assessed by site-directed mutagenesis of the coding sequence to eliminate one or the other or both of the glycosylation sites. One oligosaccharide at either position was sufficient for cell surface expression of G protein in transfected cells, and the rates of oligosaccharide processing were similar to the rate observed for wild-type protein. However, the nonglycosylated G protein synthesized when both glycosylation sites were eliminated did not reach the cell surface. This protein did appear to reach a Golgi-like region, as determined by indirect immunofluorescence microscopy, however, and was modified with palmitic acid. It was also apparently not subject to increased proteolytic breakdown.

MeSH Terms
Animals Cell Line Cell Membrane/metabolism Chlorocebus aethiops Cloning, Molecular DNA/metabolism Kidney Membrane Glycoproteins Mutation Oligosaccharides/metabolism Phosphorylation Protein Processing, Post-Translational Vesicular stomatitis Indiana virus/genetics,metabolism Viral Envelope Proteins Viral Proteins/genetics,metabolism
Chemicals
G protein, vesicular stomatitis virus Membrane Glycoproteins Oligosaccharides Viral Envelope Proteins Viral Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Machamer C E
Florkiewicz R Z
Rose J K
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.11.3074-3083.1985
Published
1985-11-00
Pages
3074-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369121
Subset
IM
Grants
NIAID NIH HHS · AI-15481 · United States
NCI NIH HHS · CA-14195 · United States
NIGMS NIH HHS · GM-33840 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.11.3074-3083.1985 PMC Full Text View journal details
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