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PMID: 3018546 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recombination hot spot in the human beta-globin gene cluster: meiotic recombination of human DNA fragments in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 5 ·No. 8 ·1985-08-00 ·Pages 2029-38

Treco D, Thomas B, Arnheim N

Abstract

We describe a novel system for the analysis of sequence-specific meiotic recombination in Saccharomyces cerevisiae. A comparison of three adjacent restriction fragments from the human beta-globin locus revealed that one of them, previously hypothesized to contain a relative hot spot for genetic recombination, engages in reciprocal exchange during yeast meiosis significantly more frequently than either of the other two fragments. Removal of the longest of four potential Z-DNA-forming regions from this fragment does not affect the high frequency of genetic recombination.

MeSH Terms
Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/analysis Genes Globins/genetics Homozygote Humans Meiosis Plasmids Recombination, Genetic Saccharomyces cerevisiae/cytology,genetics
Chemicals
DNA, Recombinant Globins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Treco D
Thomas B
Arnheim N
References (51)
51 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.8.2029-2038.1985
Published
1985-08-00
Pages
2029-38
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366921
Subset
IM
Grants
NIGMS NIH HHS · GM-08065 · United States
NIGMS NIH HHS · GM-25365 · United States
NIGMS NIH HHS · GM-28632 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.8.2029-2038.1985 PMC Full Text View journal details
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