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PMID: 6220942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of nonhomology on bacteriophage lambda recombination.

Genetics ·Vol. 103 ·No. 1 ·1983-01-00 ·Pages 5-22

Lichten M, Fox MS

Abstract

When crosses are performed under conditions severely restricting DNA synthesis, the presence of DNA sequence nonhomologies in the lac region of lambda plac5 limits the parental material contribution to and the yield of phage recombinant in a region bracketed by the nonhomologies. These observations are consistent with the expectation of a role for branch migration in the formation of heteroduplex structures under these conditions. Under conditions permissive for DNA replication, bracketing a region with nonhomologies has an only modest effect on the yield of recombinants within that interval. In addition, recombinants within such a bracketed interval manifest an excess of coincident exchange events in an adjacent region. These observations suggest the possibility that, under conditions permissive for DNA replication, regions of nonhomology can be included in heteroduplex structures.

MeSH Terms
Bacteriophage lambda/genetics DNA Replication DNA, Viral/genetics Models, Genetic Recombination, Genetic Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lichten M
Fox M S
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33 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/103.1.5
Published
1983-01-00
Pages
5-22
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1202024
Subset
IM
Grants
NIAID NIH HHS · AI05388 · United States
NIGMS NIH HHS · GM07287 · United States
External Links
PubMed source DOI: 10.1093/genetics/103.1.5 PMC Full Text View journal details
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