Home LiteratureArticle Details
PMID: 3018667 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga.

Nucleic acids research ·Vol. 14 ·No. 15 ·1986-08-11 ·Pages 6017-30

Xia Y, Burbank DE, Van Etten JL

Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

MeSH Terms
Base Sequence Chlorella/enzymology DNA/metabolism DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes/isolation & purification,metabolism DNA Viruses/physiology DNA, Viral/metabolism Deoxyribonucleases, Type II Site-Specific Enzyme Induction Methylation Plant Viruses/physiology
Chemicals
DNA, Viral DNA DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GANTC-specific type II deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Xia Y
Burbank D E
Van Etten J L
References (10)
10 references, click to expand
  1. The in vitro restriction of the replicative form of W.T. and mutant fd phage DNA.
    Virology. 1971 Dec;46(3):703-10 PMID: 4944861
  2. Purification and properties of a new restriction endonuclease from Haemophilus influenzae Rf.
    Eur J Biochem. 1978 Dec;92(2):417-26 PMID: 33045
  3. Assaying of organisms for the presence of restriction endonucleases.
    Methods Enzymol. 1980;65(1):19-23 PMID: 6246341
  4. Insertion mutations in the dam gene of Escherichia coli K-12.
    Mol Gen Genet. 1983;192(1-2):288-9 PMID: 6316110
  5. DNA methyltransferase induced by PBCV-1 virus infection of a Chlorella-like green alga.
    Mol Cell Biol. 1986 May;6(5):1440-5 PMID: 3537703
  6. The effect of site specific methylation on restriction endonuclease digestion.
    Nucleic Acids Res. 1985;13 Suppl:r201-7 PMID: 2987886
  7. DNA methylation of viruses infecting a eukaryotic Chlorella-like green alga.
    Nucleic Acids Res. 1985 May 24;13(10):3471-8 PMID: 4011432
  8. Characterization of viruses infecting a eukaryotic Chlorella-like green alga.
    Virology. 1986 Apr 15;150(1):170-7 PMID: 3006334
  9. Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga.
    Mol Cell Biol. 1986 May;6(5):1430-9 PMID: 3023890
  10. Lytic viruses infecting a Chlorella-like alga.
    Virology. 1985 Jan 15;140(1):135-43 PMID: 2981448
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.15.6017
Published
1986-08-11
Pages
6017-30
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311618
Subset
IM
Grants
NIGMS NIH HHS · GM-32441 · United States
External Links
PubMed source DOI: 10.1093/nar/14.15.6017 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2