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PMID: 3018731 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Inositol cyclic triphosphate [inositol 1,2-(cyclic)-4,5-triphosphate] is formed upon thrombin stimulation of human platelets.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6397-401

Ishii H, Connolly TM, Bross TE, Majerus PW

Abstract

Cleavage of polyphosphoinositides in vitro by phospholipase C results in formation of both cyclic and noncyclic inositol phosphates. We have now isolated the cyclic product of phosphatidylinositol 4,5-bisphosphate cleavage, inositol 1,2(cyclic)-4,5-triphosphate [cIns(1:2,4,5)P3], from thrombin-treated platelets. We found 0.2-0.4 nmol of cIns-(1:2,4,5)P3 per 10(9) platelets at 10 sec after thrombin; none was found in unstimulated platelets or in platelets 10 min after thrombin addition. We conclude that cIns(1:2,4,5)P3 is a major product of polyphosphoinositide metabolism in thrombin-stimulated platelets.

MeSH Terms
Blood Platelets/metabolism Chromatography, High Pressure Liquid Humans Inositol Phosphates/blood Phosphatidylinositols/blood Sugar Phosphates/blood Thrombin/metabolism
Chemicals
Inositol Phosphates Phosphatidylinositols Sugar Phosphates inositol-1,2-cyclic-4,5-triphosphate Thrombin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ishii H
Connolly T M
Bross T E
Majerus P W
References (20)
20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6397
Published
1986-09-00
Pages
6397-401
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386510
Subset
IM
Grants
NHLBI NIH HHS · HL 16634 · United States
NHLBI NIH HHS · HLBI 14147 · United States
NHLBI NIH HHS · T32 HLBI 07088 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.17.6397 PMC Full Text View journal details
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