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PMID: 30208757 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Autophagy regulates glucose-mediated root meristem activity by modulating ROS production in Arabidopsis.

Autophagy ·Vol. 15 ·No. 3 ·2019-00-00 ·Pages 407-422

Huang L, Yu LJ, Zhang X, Fan B, Wang FZ, Dai YS, Qi H, Zhou Y, Xie LJ, Xiao S

Abstract

Glucose produced from photosynthesis is a key nutrient signal regulating root meristem activity in plants; however, the underlying mechanisms remain poorly understood. Here, we show that, by modulating reactive oxygen species (ROS) levels, the conserved macroautophagy/autophagy degradation pathway contributes to glucose-regulated root meristem maintenance. In Arabidopsis thaliana roots, a short exposure to elevated glucose temporarily suppresses constitutive autophagosome formation. The autophagy-defective autophagy-related gene (atg) mutants have enhanced tolerance to glucose, established downstream of the glucose sensors, and accumulate less glucose-induced ROS in the root tips. Moreover, the enhanced root meristem activities in the atg mutants are associated with improved auxin gradients and auxin responses. By acting with AT4G39850/ABCD1 (ATP-binding cassette D1; Formerly PXA1/peroxisomal ABC transporter 1), autophagy plays an indispensable role in the glucose-promoted degradation of root peroxisomes, and the atg mutant phenotype is partially rescued by the overexpression of ABCD1. Together, our findings suggest that autophagy is an essential mechanism for glucose-mediated maintenance of the root meristem. Abbreviation: ABA: abscisic acid; ABCD1: ATP-binding cassette D1; ABO: ABA overly sensitive; AsA: ascorbic acid; ATG: autophagy related; CFP: cyan fluorescent protein; Co-IP: co-immunoprecipitation; DAB: 3',3'-diaininobenzidine; DCFH-DA: 2',7'-dichlorodihydrofluorescin diacetate; DR5: a synthetic auxin response element consists of tandem direct repeats of 11 bp that included the auxin-responsive TGTCTC element; DZ: differentiation zone; EZ, elongation zone; GFP, green fluorescent protein; GSH, glutathione; GUS: β-glucuronidase; HXK1: hexokinase 1; H2O2: hydrogen peroxide; IAA: indole-3-acetic acid; IBA: indole-3-butyric acid; KIN10/11: SNF1 kinase homolog 10/11; MDC: monodansylcadaverine; MS: Murashige and Skoog; MZ: meristem zone; NBT: nitroblue tetrazolium; NPA: 1-N-naphtylphthalamic acid; OxIAA: 2-oxindole-3-acetic acid; PIN: PIN-FORMED; PLT: PLETHORA; QC: quiescent center; RGS1: Regulator of G-protein signaling 1; ROS: reactive oxygen species; SCR: SCARECROW; SHR, SHORT-ROOT; SKL: Ser-Lys-Leu; SnRK1: SNF1-related kinase 1; TOR: target of rapamycin; UPB1: UPBEAT1; WOX5: WUSCHEL related homeobox 5; Y2H: yeast two-hybrid; YFP: yellow fluorescent protein.

Keywords
Autophagy glucose peroxisome reactive oxygen species root meristem
MeSH Terms
ATP Binding Cassette Transporter, Subfamily D, Member 1/metabolism Arabidopsis/genetics,metabolism Arabidopsis Proteins/metabolism Autophagosomes/metabolism Autophagy/drug effects,genetics Autophagy-Related Proteins/genetics,metabolism Gene Expression Regulation, Plant Glucose/pharmacology Indoleacetic Acids/metabolism Meristem/metabolism Peroxisomes/metabolism Plant Roots/genetics,metabolism Reactive Oxygen Species/metabolism Signal Transduction/drug effects,genetics
Chemicals
ATP Binding Cassette Transporter, Subfamily D, Member 1 Arabidopsis Proteins Autophagy-Related Proteins Indoleacetic Acids Reactive Oxygen Species Glucose
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Huang Li
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Yu Lu-Jun
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Zhang Xue
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Fan Biao
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Wang Feng-Zhu
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Dai Yang-Shuo
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Qi Hua
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Zhou Ying
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Xie Li-Juan
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
Xiao Shi
a State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences , Sun Yat-sen University , Guangzhou , China.
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Article Info
Journal
Autophagy
Abbr.
Autophagy
ISSN
1554-8635
DOI
10.1080/15548627.2018.1520547
Published
2019-00-00
Epub
2018-00-22
Pages
407-422
Language
English
Region
United States
NLM ID
101265188
PMCID
PMC6351127
Subset
IM
External Links
PubMed source DOI: 10.1080/15548627.2018.1520547 PMC Full Text View journal details
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