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PMID: 23667126 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The α/β hydrolase CGI-58 and peroxisomal transport protein PXA1 coregulate lipid homeostasis and signaling in Arabidopsis.

The Plant cell ·Vol. 25 ·No. 5 ·2013-05-00 ·Pages 1726-39

Park S, Gidda SK, James CN, Horn PJ, Khuu N, Seay DC, Keereetaweep J, Chapman KD, Mullen RT, Dyer JM

Abstract

COMPARATIVE GENE IDENTIFICATION-58 (CGI-58) is a key regulator of lipid metabolism and signaling in mammals, but its underlying mechanisms are unclear. Disruption of CGI-58 in either mammals or plants results in a significant increase in triacylglycerol (TAG), suggesting that CGI-58 activity is evolutionarily conserved. However, plants lack proteins that are important for CGI-58 activity in mammals. Here, we demonstrate that CGI-58 functions by interacting with the PEROXISOMAL ABC-TRANSPORTER1 (PXA1), a protein that transports a variety of substrates into peroxisomes for their subsequent metabolism by β-oxidation, including fatty acids and lipophilic hormone precursors of the jasmonate and auxin biosynthetic pathways. We also show that mutant cgi-58 plants display changes in jasmonate biosynthesis, auxin signaling, and lipid metabolism consistent with reduced PXA1 activity in planta and that, based on the double mutant cgi-58 pxa1, PXA1 is epistatic to CGI-58 in all of these processes. However, CGI-58 was not required for the PXA1-dependent breakdown of TAG in germinated seeds. Collectively, the results reveal that CGI-58 positively regulates many aspects of PXA1 activity in plants and that these two proteins function to coregulate lipid metabolism and signaling, particularly in nonseed vegetative tissues. Similarities and differences of CGI-58 activity in plants versus animals are discussed.

MeSH Terms
ATP-Binding Cassette Transporters/genetics,metabolism Acyltransferases/genetics,metabolism Adenosine Triphosphatases Amino Acid Sequence Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Cell Nucleus/metabolism Cyclopentanes/metabolism Fatty Acid Transport Proteins/genetics,metabolism Fatty Acids/metabolism Homeostasis Hydrolases/genetics,metabolism Indoleacetic Acids/metabolism,pharmacology Lipid Metabolism Luminescent Proteins/genetics,metabolism Microscopy, Confocal Molecular Sequence Data Mutation Oxylipins/metabolism Peroxisomes/metabolism Plant Growth Regulators/metabolism,pharmacology Plant Roots/drug effects,genetics,growth & development Protein Binding Seeds/genetics,growth & development,metabolism Sequence Homology, Amino Acid Signal Transduction Triglycerides/metabolism Two-Hybrid System Techniques
Chemicals
ATP-Binding Cassette Transporters Arabidopsis Proteins Cyclopentanes Fatty Acid Transport Proteins Fatty Acids Indoleacetic Acids Luminescent Proteins Oxylipins Plant Growth Regulators Triglycerides jasmonic acid Acyltransferases 2-acylglycerophosphate acyltransferase Hydrolases Adenosine Triphosphatases At4g39850 protein, Arabidopsis
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Park Sunjung
U.S. Department of Agriculture-Agricultural Research Service, U.S. Arid-Land Agricultural Research Center, Maricopa, Arizona 85138, USA.
Gidda Satinder K
James Christopher N
Horn Patrick J
Khuu Nicholas
Seay Damien C
Keereetaweep Jantana
Chapman Kent D
Mullen Robert T
Dyer John M
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.113.111898
Published
2013-05-00
Epub
2013-00-10
Pages
1726-39
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3694702
Subset
IM
Corrections
CommentIn
PMID: 23667125
External Links
PubMed source DOI: 10.1105/tpc.113.111898 PMC Full Text View journal details
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