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PMID: 3020940 Published · ppublish English Journal Article

Characterization of macrophages elicited by intraperitoneal injection of hyaluronate.

Agents and actions ·Vol. 18 ·No. 5-6 ·1986-08-00 ·Pages 544-9

Ponzin D, Vecchia P, Toffano G, Giordano C, Bruni A

Abstract

Hyaluronate of 120,000 molecular weight has been injected in the peritoneal cavity of mice to study its effect on migration of inflammatory cells in vivo. After one day a dose-dependent granulocyte migration is observed. Three days later the number of granulocytes is greatly reduced and macrophages form about half of the total cell population. Hyaluronate-elicited macrophages show a decreased 5'-nucleotidase and an increased acid phosphatase activity as compared to resident macrophages. The production of superoxide anion in response to the phorbol ester tetradecanoyl-phorbolacetate, and the phagocytic activity are also enhanced. Macrophages elicited by hyaluronate secrete growth factor(s) for non-lymphoid mesenchymal cells. It is concluded that hyaluronate in vivo stimulates the migration of inflammatory cells, thus causing the recruitment of a population of stimulating macrophages. These effects may explain previous reports on the acceleration of wound healing by hyaluronate.

MeSH Terms
5'-Nucleotidase Acid Phosphatase/metabolism Animals Cell Division Hyaluronic Acid/administration & dosage,pharmacology Injections, Intraperitoneal Kinetics Macrophages/cytology,enzymology,physiology Male Mice Nucleotidases/metabolism Phagocytosis
Chemicals
Hyaluronic Acid Nucleotidases Acid Phosphatase 5'-Nucleotidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ponzin D
Vecchia P
Toffano G
Giordano C
Bruni A
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23 references, click to expand
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Article Info
Journal
Agents and actions
Abbr.
Agents Actions
ISSN
0065-4299
DOI
10.1007/BF01964962
Published
1986-08-00
Pages
544-9
Language
English
Region
Switzerland
NLM ID
0213341
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01964962
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