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PMID: 3021817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Recombinant human granulocyte-macrophage colony-stimulating factor stimulates in vitro mature human neutrophil and eosinophil function, surface receptor expression, and survival.

The Journal of clinical investigation ·Vol. 78 ·No. 5 ·1986-11-00 ·Pages 1220-8

Lopez AF, Williamson DJ, Gamble JR, Begley CG, Harlan JM, Klebanoff SJ, Waltersdorph A, Wong G, Clark SC, Vadas MA

Abstract

A purified recombinant human granulocyte-macrophage colony stimulating factor (rH GM-CSF) was a powerful stimulator of mature human eosinophils and neutrophils. The purified rH GM-CSF enhanced the cytotoxic activity of neutrophils and eosinophils against antibody-coated targets, stimulated phagocytosis of serum-opsonized yeast by both cell types in a dose-dependent manner, and stimulated neutrophil-mediated iodination in the presence of zymosan. In addition, rH GM-CSF enhanced N-formylmethionylleucylphenylalanine(FMLP)-stimulated degranulation of Cytochalasin B pretreated neutrophils and FMLP-stimulated superoxide production. In contrast, rH GM-CSF did not promote adherence of granulocytes to endothelial cells or plastic surfaces. rH GM-CSF selectively enhanced the surface expression of granulocyte functional antigens 1 and 2, and the Mo1 antigen. rH GM-CSF induced morphological changes and enhanced the survival of both neutrophils and eosinophils by 6 and 9 h, respectively. These experiments show that granulocyte-macrophage colony stimulating factor can selectively stimulate mature granulocyte function.

MeSH Terms
Antibody-Dependent Cell Cytotoxicity Cell Adhesion/drug effects Cell Survival/drug effects Cytoplasmic Granules/ultrastructure Eosinophils/cytology,immunology,physiology Flow Cytometry Humans Interleukin-3/physiology Neutrophils/cytology,immunology,physiology Phagocytosis Recombinant Proteins/pharmacology Superoxides/blood
Chemicals
Interleukin-3 Recombinant Proteins Superoxides
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Lopez A F
Williamson D J
Gamble J R
Begley C G
Harlan J M
Klebanoff S J
Waltersdorph A
Wong G
Clark S C
Vadas M A
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44 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112705
Published
1986-11-00
Pages
1220-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC423807
Subset
IM
Grants
NIAID NIH HHS · AI-07763 · United States
NIAID NIH HHS · AI-21876 · United States
External Links
PubMed source DOI: 10.1172/JCI112705 PMC Full Text View journal details
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