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PMID: 2993312 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Release of superoxide and change in morphology by neutrophils in response to phorbol esters: antagonism by inhibitors of calcium-binding proteins.

The Journal of cell biology ·Vol. 101 ·No. 3 ·1985-09-00 ·Pages 1052-8

Robinson JM, Badwey JA, Karnovsky ML, Karnovsky MJ

Abstract

The ability of phorbol derivatives to function as stimulating agents for superoxide (O2-) release by guinea pig neutrophils has been evaluated and compared to the known ability of each compound to activate protein kinase C. Those that activate the kinase also stimulate O2- release, while those that are inactive with respect to the kinase have no effect on O2- release. The same correlation was observed with respect to the ability of phorbol esters to induce morphological changes in neutrophils, i.e., vesiculation and reduction in granule content. Certain phenothiazines and naphthalene sulfonamides that are known antagonists of calcium-binding proteins blocked both phorbol ester-induced O2- release and morphological changes in these cells.

MeSH Terms
Animals Calcium-Binding Proteins/antagonists & inhibitors Calmodulin/antagonists & inhibitors Guinea Pigs Microscopy, Electron Neutrophils/drug effects,metabolism,ultrastructure Phenothiazines/pharmacology Phorbol Esters/pharmacology Phorbols/pharmacology Protein Kinase C Protein Kinase Inhibitors Sulfonamides/pharmacology Superoxides/metabolism
Chemicals
Calcium-Binding Proteins Calmodulin Phenothiazines Phorbol Esters Phorbols Protein Kinase Inhibitors Sulfonamides Superoxides Protein Kinase C
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Robinson J M
Badwey J A
Karnovsky M L
Karnovsky M J
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43 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.101.3.1052
Published
1985-09-00
Pages
1052-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113709
Subset
IM
Grants
NIAID NIH HHS · AI 03260 · United States
NIAID NIH HHS · AI 17945 · United States
External Links
PubMed source DOI: 10.1083/jcb.101.3.1052 PMC Full Text View journal details
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