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PMID: 3022008 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complex formation of simian virus 40 large T antigen with cellular protein p53.

Journal of virology ·Vol. 60 ·No. 2 ·1986-11-00 ·Pages 761-4

Montenarh M, Kohler M, Henning R

Abstract

We investigated the formation of native complexes between simian virus 40 large T antigen and the cellular protein p53 (T-p53) by using simian virus 40 tsA58-transformed mouse fibroblasts (tsA58 F2b). We observed that newly synthesized p53 bound to all structural subclasses of large T antigen detectable on sucrose density gradients. This led to various intermediates of T-p53 complexes which converted within 2 h into typical mature aggregates. The final levels of stable T-p53 complexes seemed to be determined by p53 rather than by large T antigen.

MeSH Terms
Animals Antigens, Viral, Tumor Cell Line Cell Transformation, Viral Mice Neoplasm Proteins/metabolism Phosphoproteins/metabolism Phosphorylation Simian virus 40/immunology Temperature Tumor Suppressor Protein p53
Chemicals
Antigens, Viral, Tumor Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Montenarh M
Kohler M
Henning R
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.2.761-764.1986
Published
1986-11-00
Pages
761-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288952
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.60.2.761-764.1986 PMC Full Text View journal details
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