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PMID: 3023398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Glucocorticoid-regulated glycoprotein maturation in wild-type and mutant rat cell lines.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 1 ·1986-12-00 ·Pages 2323-31

Firestone GL, John NJ, Yamamoto KR

Abstract

Glucocorticoid hormones can regulate the posttranslational maturation of mouse mammary tumor virus (MTV) precursor polyproteins in M1.54, a stably infected rat hepatoma cell line. We have used complement-mediated cytolysis to recover variants of M1.54 that fail to express MTV cell surface glycoproteins in a hormone-regulated manner (Firestone, G.L., and K.R. Yamamoto, 1983, Mol. Cell. Biol., 3:149-160). One such clonal isolate, CR4, is similar to wild-type with respect to synthesis of MTV mRNAs, production of the MTV glycoprotein precursor (gPr74env) and a glycosylated maturation product (gp51), and hormone-induced processing of two MTV phosphoproteins. In contrast, three viral cell surface glycoproteins (gp78, gp70, and gp32) and one extracellular species (gp70s), which derive from gPr74env in glucocorticoid-treated wild-type cells, fail to appear in CR4. CR4 showed no apparent alterations in proliferation rate, cell shape, or expression of total functional mRNA and bulk glycoproteins. We conclude that the genetic lesion in CR4 defines a highly selective hormone-regulated glycoprotein maturation pathway that alters the fate of a restricted subset of precursor species.

MeSH Terms
Animals Cell Division/drug effects Cell Line Dexamethasone/pharmacology Glycoproteins/genetics Liver Neoplasms, Experimental Mammary Tumor Virus, Mouse/genetics Membrane Proteins/genetics Mutation Protein Biosynthesis Protein Processing, Post-Translational/drug effects RNA, Messenger/genetics Rats Viral Proteins/genetics
Chemicals
Glycoproteins Membrane Proteins RNA, Messenger Viral Proteins Dexamethasone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Firestone G L
John N J
Yamamoto K R
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63 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2323
Published
1986-12-00
Pages
2323-31
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114581
Subset
IM
Grants
NCI NIH HHS · CA 09041 · United States
NCI NIH HHS · CA 20535 · United States
NCI NIH HHS · CA 35547 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2323 PMC Full Text View journal details
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