Human cDNA clones for four species of G alpha s signal transduction protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 23 ·1986-12-00 ·Pages 8893-7

Bray P, Carter A, Simons C, Guo V, Puckett C, Kamholz J, Spiegel A, Nirenberg M

Abstract

lambda gt11 cDNA libraries derived from human brain were screened with oligonucleotide probes for recombinants that code for alpha subunits of G signal transduction proteins. Eleven alpha s clones were detected with both probes and characterized. Four types of alpha s cDNA were cloned that differ in nucleotide sequence in the region that corresponds to amino acid residues 71-88. The clones differ in the codon for alpha s amino acid residue 71 (glutamic acid or aspartic acid), the presence or absence of codons for the next 15 amino acid residues, and the presence or absence of an adjacent serine residue. S1 nuclease protection experiments revealed at least two forms of alpha s mRNA. A mechanism for generating four species of alpha s mRNA by alternative splicing of precursor RNA is proposed.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics Endonucleases Exons GTP-Binding Proteins/genetics Humans Introns RNA Splicing RNA, Messenger/genetics Single-Strand Specific DNA and RNA Endonucleases

Chemicals

RNA, Messenger DNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases GTP-Binding Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Bray P

Carter A

Simons C

Guo V

Puckett C

Kamholz J

Spiegel A

Nirenberg M

References (26)

26 references, click to expand

Action of single-strand specific nucleases on model DNA heteroduplexes of defined size and sequence.
Biochemistry. 1977 May 31;16(11):2374-9 PMID: 301043
Immunochemical evidence for a novel pertussis toxin substrate in human neutrophils.
J Biol Chem. 1986 Jun 15;261(17):8058-62 PMID: 3711124
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Empirical predictions of protein conformation.
Annu Rev Biochem. 1978;47:251-76 PMID: 354496
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
Analysis of 5' flanking sequences and intron-exon boundaries of the rat prolactin gene.
J Biol Chem. 1981 Oct 25;256(20):10524-8 PMID: 6270114
The regulatory component of adenylate cyclase. Purification and properties.
J Biol Chem. 1981 Nov 25;256(22):11517-26 PMID: 6271754
Structure and expression in L-cells of a cloned H4 histone gene of the mouse.
J Mol Biol. 1981 Oct 5;151(4):607-25 PMID: 6276563
Regulation of HIS4-lacZ fusions in Saccharomyces cerevisiae.
Mol Cell Biol. 1982 Oct;2(10):1212-9 PMID: 6817079
G proteins and dual control of adenylate cyclase.
Cell. 1984 Mar;36(3):577-9 PMID: 6321035
Homologies between signal transducing G proteins and ras gene products.
Science. 1984 Nov 16;226(4676):860-2 PMID: 6436980
Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain.
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3776-80 PMID: 3086867
Molecular basis for two forms of the G protein that stimulates adenylate cyclase.
J Biol Chem. 1986 Jul 25;261(21):9587-90 PMID: 3015900
Splicing of messenger RNA precursors.
Annu Rev Biochem. 1986;55:1119-50 PMID: 2943217
Identification of specific transducin alpha subunits in retinal rod and cone photoreceptors.
Science. 1986 Oct 3;234(4772):77-80 PMID: 3529395
Guanine nucleotide-sensitive, high affinity binding of carbachol to muscarinic cholinergic receptors of 1321N1 astrocytoma cells is insensitive to pertussis toxin.
Mol Pharmacol. 1985 Jan;27(1):32-7 PMID: 2981400
Sequence of the alpha subunit of photoreceptor G protein: homologies between transducin, ras, and elongation factors.
Science. 1985 Apr 5;228(4695):96-9 PMID: 3856323
Primary structure of the alpha-subunit of transducin and its relationship to ras proteins.
Nature. 1985 May 16-22;315(6016):242-5 PMID: 3923359
Amino acid sequence of the alpha subunit of transducin deduced from the cDNA sequence.
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4311-5 PMID: 2409555
Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase.
Science. 1985 Sep 20;229(4719):1274-7 PMID: 3839937
Studies on the phosphorylation of myelin basic protein by protein kinase C and adenosine 3':5'-monophosphate-dependent protein kinase.
J Biol Chem. 1985 Oct 15;260(23):12492-9 PMID: 2413024
GTP-binding proteins couple cardiac muscarinic receptors to a K channel.
Nature. 1985 Oct 10-16;317(6037):536-8 PMID: 2413367
Primary structure of the alpha-subunit of bovine adenylate cyclase-stimulating G-protein deduced from the cDNA sequence.
FEBS Lett. 1986 Jan 20;195(1-2):220-4 PMID: 3080331
Primary structure of the alpha-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence.
FEBS Lett. 1986 Mar 3;197(1-2):305-10 PMID: 2419165
Deduced primary structure of the alpha subunit of the GTP-binding stimulatory protein of adenylate cyclase.
Proc Natl Acad Sci U S A. 1986 Mar;83(5):1251-5 PMID: 3081893
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
Cell. 1977 Nov;12(3):721-32 PMID: 922889

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.83.23.8893

Published

1986-12-00

Pages

8893-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387039

Subset

Databases

GENBANK

M14631

External Links

PubMed source DOI: 10.1073/pnas.83.23.8893 PMC Full Text View journal details

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Human cDNA clones for four species of G alpha s signal transduction protein.

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