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PMID: 3025219 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Topoisomerase II: A specific marker for cell proliferation.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 2 ·1986-12-00 ·Pages 2569-81

Heck MM, Earnshaw WC

Abstract

We have used an antibody probe to measure the levels of topoisomerase II in several transformed and developmentally regulated normal cell types. Transformed cells contain roughly 1 X 10(6) copies of the enzyme. During erythropoiesis in chicken embryos the enzyme level drops from 7.8 X 10(4) copies per erythroblast to less than 300 copies per erythrocyte concomitant with the cessation of mitosis in the blood. Cultured myoblasts also lose topoisomerase II upon fusion into nonproliferating myotubes. When peripheral blood lymphocytes (which lack detectable topoisomerase II) commence proliferation, they express topoisomerase II de novo. Appearance of the enzyme exactly parallels the onset of DNA replication. These results suggest that topoisomerase II is not required for transcription in higher eukaryotes, but that it may function during DNA replication. Furthermore, topoisomerase II is a sensitive and specific marker for proliferating cells.

MeSH Terms
Animals Cell Division Cell Line Chick Embryo DNA Replication DNA Topoisomerases, Type II/immunology,metabolism Erythrocytes/enzymology Erythropoiesis Immunologic Techniques Lymphocyte Activation Muscles/cytology,enzymology Transcription, Genetic
Chemicals
DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heck M M
Earnshaw W C
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50 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2569
Published
1986-12-00
Pages
2569-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114590
Subset
IM
Grants
NIGMS NIH HHS · GM-30985 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2569 PMC Full Text View journal details
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