Home LiteratureArticle Details
PMID: 6327265 Published · ppublish English Journal Article

Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.

The EMBO journal ·Vol. 1 ·No. 11 ·1982-00-00 ·Pages 1353-8

Luchnik AN, Bakayev VV, Zbarsky IB, Georgiev GP

Abstract

After treatment of SV40 minichromosomes with DNA topoisomerase I, the superhelicity in the bulk of the DNA extracted from minichromosomes is known to remain unchanged. However, we found that the DNA extracted from a small fraction of SV40 minichromosomes (2-5%), was almost completely relaxed, and covalently closed as shown by agarose gel electrophoresis. Thus, the DNA in these 2-5% of SV40 minichromosomes was probably torsionally strained (TS). The proportion of such TS minichromosomes is close to the estimated proportion of transcriptionally active minichromosomes. The distribution of the TS minichromosomes in sucrose gradient coincided with the distribution of transcriptionally active complexes. Both sedimented faster than the majority of minichromosomes. Furthermore, after treatment with topoisomerase I the relaxed minichromosomes could be quantitatively separated from the bulk of material by recentrifugation in a sucrose gradient. A major part of the endogenous RNA polymerase activity was recovered in the relaxed fraction. These data suggest that TS-minichromosomes correspond to transcriptionally active chromatin. After relaxation with topoisomerase I the TS minichromosomes lacked histones.

MeSH Terms
Animals Cell Line Chlorocebus aethiops Chromosomes/ultrastructure DNA/analysis DNA Topoisomerases, Type I DNA, Viral/analysis,genetics Elasticity Kidney Simian virus 40/genetics Transcription, Genetic
Chemicals
DNA, Viral DNA DNA Topoisomerases, Type I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Luchnik A N
Bakayev V V
Zbarsky I B
Georgiev G P
References (38)
38 references, click to expand
  1. SV40-specific nucleoprotein complexes: heterogeneity and composition.
    Virology. 1981 Mar;109(2):244-56 PMID: 6259808
  2. Decrease in the number of DNA topological turns during Friend erythroleukemia differentiation.
    Mol Gen Genet. 1980;178(2):459-63 PMID: 6930535
  3. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51 PMID: 6265907
  4. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  5. Deletion mapping a eukaryotic promoter.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4461-5 PMID: 7027262
  6. Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3304-8 PMID: 226966
  7. Effect of nalidixic acid and novobiocin on pBR322 genetic expression in Escherichia coli minicells.
    J Bacteriol. 1981 Dec;148(3):745-52 PMID: 7031033
  8. The interaction of RNA polymerase II with non-promoter DNA sites.
    Nucleic Acids Res. 1981 Nov 25;9(22):6031-46 PMID: 6273819
  9. A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication.
    Nucleic Acids Res. 1981 Dec 11;9(23):6231-50 PMID: 6275353
  10. A small segment of polyoma virus DNA enhances the expression of a cloned beta-globin gene over a distance of 1400 base pairs.
    Nucleic Acids Res. 1981 Dec 11;9(23):6251-64 PMID: 6275354
  11. Different chromatin structures in Physarum polycephalum: a special form of transcriptionally active chromatin devoid of nucleosomal particles.
    Chromosoma. 1981;84(2):279-90 PMID: 7327047
  12. DNA topological linking numbers in malignantly transformed Syrian hamster cells.
    Mol Gen Genet. 1981;183(3):553-6 PMID: 6950190
  13. Active chromatin.
    Nature. 1982 May 27;297(5864):289-95 PMID: 6210847
  14. Decrease in the density of DNA topological turns during vitro aging of Syrian hamster cells.
    Exp Cell Res. 1982 Jun;139(2):249-55 PMID: 6177540
  15. Heterogeneity of deoxynucleoprotein particles as evidencec by ultracentrifugation of cesium chloride density gradient.
    J Mol Biol. 1969 Apr;41(2):299-303 PMID: 5805452
  16. Animal DNA-dependent RNA polymerases. Studies on the reaction parameters of transcription in vitro of Simian virus 40 DNA by mammalian RNA polymerases AI and B.
    Eur J Biochem. 1974 Jan 16;41(2):367-78 PMID: 4361417
  17. Animal DNA-dependent RNA polymerases. Studies on the binding of mammalian RNA polymerases AI and B to Simian virus 40 DNA.
    Eur J Biochem. 1974 Jan 16;41(2):397-411 PMID: 4361419
  18. SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNA.
    Nature. 1974 May 24;249(455):343-4 PMID: 4366777
  19. Initiation of transcription by Escherichia coli RNA polymerase from supercoiled and non-supercoiled bacteriophage PM2 DNA.
    J Mol Biol. 1975 Feb 5;91(4):477-87 PMID: 1097698
  20. Conformational transitions in closed circular DNA molecules. II. Biological implications.
    Mol Biol Rep. 1980 Mar 31;6(1):11-5 PMID: 7393222
  21. Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells.
    Cell. 1980 Oct;21(3):773-83 PMID: 6254668
  22. Extraction of transcribing SV40 chromosomes in very low salt.
    Nucleic Acids Res. 1980 Feb 11;8(3):573-86 PMID: 6255446
  23. Structure of transcribing chromatin.
    Prog Nucleic Acid Res Mol Biol. 1980;24:1-55 PMID: 6256821
  24. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6 PMID: 6938957
  25. Folding of the DNA double helix in chromatin-like structures from simian virus 40.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 PMID: 168578
  26. The number of superhelical turns in native virion SV40 DNA and minicol DNA determined by the band counting method.
    Cell. 1976 Jun;8(2):215-26 PMID: 183892
  27. Comparative organization of active transcription units in Oncopeltus fasciatus.
    Cell. 1976 Sep;9(1):131-46 PMID: 975237
  28. An electron microscopic comparison of transcription on linear and superhelical DNA.
    J Mol Biol. 1976 Jul 25;105(1):161-76 PMID: 792457
  29. The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.
    Nucleic Acids Res. 1977 Dec;4(12):4261-77 PMID: 202928
  30. Structure and reactions of closed duplex DNA.
    Annu Rev Biophys Bioeng. 1978;7:287-313 PMID: 208457
  31. Novel template requirements of N4 virion RNA polymerase.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3220-4 PMID: 356050
  32. Promoter-specific inhibition of transcription by antibiotics which act on DNA gyrase.
    Nature. 1978 Oct 5;275(5679):420-3 PMID: 211433
  33. Purification of a DNA nicking-closing enzyme from mouse L cells.
    Nucleic Acids Res. 1978 Aug;5(8):2861-75 PMID: 211488
  34. Transcription of polyoma virus DNA in vitro. II. Transcription of superhelical and linear polyoma DNA by RNA polymerase II.
    J Mol Biol. 1978 Sep 5;124(1):73-85 PMID: 213607
  35. The interaction of RNA polymerase II from wheat with supercoiled and linear plasmid templates.
    Nucleic Acids Res. 1979 Feb;6(2):507-23 PMID: 370789
  36. Modulation of gene expression by drugs affecting deoxyribonucleic acid gyrase.
    J Bacteriol. 1979 Apr;138(1):40-7 PMID: 108253
  37. Nicking-closing enzyme is associated with SV40 DNA in vivo as a sodium dodecyl sulfate-resistant complex.
    Nucleic Acids Res. 1979 Oct 10;7(3):679-87 PMID: 228247
  38. In vivo sequence requirements of the SV40 early promotor region.
    Nature. 1981 Mar 26;290(5804):304-10 PMID: 6259538
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1982-00-00
Pages
1353-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553216
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2