Home LiteratureArticle Details
PMID: 3025614 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mating type-like conversion promoted by the 2 micrograms circle site-specific recombinase: implications for the double-strand-gap repair model.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 3831-7

Jayaram M

Abstract

Double-strand breaks in DNA are known to promote recombination in Saccharomyces cerevisiae. Yeast mating type switching, which is a highly efficient gene conversion event, is apparently initiated by a site-specific double-strand break. The 2 micrograms circle site-specific recombinase, FLP, has been shown to make double-strand breaks in its substrate DNA. By using a hybrid 2 micrograms circle::Tn5 plasmid, a portion of which resembles, in its DNA organization, the active (MAT) and the silent (HML) yeast mating type loci, it is shown that FLP mediates a conversion event analogous to mating type switching. Whereas the FLP site-specific recombination is not dependent on the RAD52 gene product, the FLP-induced conversion is abolished in a rad52 background. The FLP-promoted conversion in vivo can be faithfully reproduced by making a double-stranded gap in vitro in the vicinity of the FLP site and allowing the gap to be repaired in vivo.

MeSH Terms
Crosses, Genetic DNA Nucleotidyltransferases/metabolism DNA Repair DNA Restriction Enzymes DNA Transposable Elements Genes, Fungal Genes, Mating Type, Fungal Plasmids Recombination, Genetic Saccharomyces cerevisiae/enzymology,genetics
Chemicals
DNA Transposable Elements DNA Nucleotidyltransferases FLP recombinase DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jayaram M
References (31)
31 references, click to expand
  1. Homothallic mating type switching generates lethal chromosome breaks in rad52 strains of Saccharomyces cerevisiae.
    Mol Cell Biol. 1981 Jun;1(6):522-34 PMID: 6765605
  2. Yeast plasmid requires a cis-acting locus and two plasmid proteins for its stable maintenance.
    Cell. 1983 Dec;35(2 Pt 1):487-93 PMID: 6317192
  3. Recombination within the yeast plasmid 2mu circle is site-specific.
    Cell. 1982 May;29(1):227-34 PMID: 6286142
  4. Determination of DNA sequences essential for FLP-mediated recombination by a novel method.
    J Biol Chem. 1985 Oct 5;260(22):12320-7 PMID: 2995371
  5. Yeast recombination: the association between double-strand gap repair and crossing-over.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4417-21 PMID: 6308623
  6. The yeast plasmid 2mu circle encodes components required for its high copy propagation.
    Cell. 1983 Aug;34(1):95-104 PMID: 6883512
  7. Isolation of a circular derivative of yeast chromosome III: implications for the mechanism of mating type interconversion.
    Cell. 1979 Oct;18(2):309-19 PMID: 387260
  8. Molecular analysis of a cell lineage.
    Nature. 1983 Apr 21;302(5910):670-6 PMID: 6339953
  9. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus.
    Cell. 1982 Nov;31(1):183-92 PMID: 6297747
  10. Isolation of yeast DNA.
    Methods Cell Biol. 1975;12:39-44 PMID: 1105072
  11. The FLP recombinase of the 2 micron circle DNA of yeast: interaction with its target sequences.
    Cell. 1985 Apr;40(4):795-803 PMID: 3879971
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Nucleotide sequence of the yeast plasmid.
    Nature. 1980 Aug 28;286(5776):860-5 PMID: 6251374
  14. Intrachromosomal gene conversion in yeast.
    Nature. 1981 Jan 15;289(5794):144-8 PMID: 7005693
  15. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  16. Characterization of the transmission during cytoductant formation of the 2 micrometers DNA plasmid from Saccharomyces.
    Mol Gen Genet. 1981;183(1):59-65 PMID: 7035822
  17. The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):503-7 PMID: 6987653
  18. Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE.
    Genetics. 1980 Jan;94(1):31-50 PMID: 17248995
  19. Yeast plasmid 2-micron circle promotes recombination within bacterial transposon Tn5.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7264-8 PMID: 6316350
  20. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  21. A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae.
    Cell. 1983 Nov;35(1):167-74 PMID: 6313222
  22. The FLP recombinase of the yeast 2-micron plasmid: characterization of its recombination site.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7270-4 PMID: 2997780
  23. Two-micrometer circle site-specific recombination: the minimal substrate and the possible role of flanking sequences.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5875-9 PMID: 3898075
  24. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  25. Lack of association between intrachromosomal gene conversion and reciprocal exchange.
    Nature. 1984 Aug 30-Sep 5;310(5980):748-53 PMID: 6088989
  26. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  27. Yeast transformation: a model system for the study of recombination.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8 PMID: 6273866
  28. Resolution of recombination intermediates generated during yeast mating type switching.
    Nature. 1984 Aug 30-Sep 5;310(5980):744-8 PMID: 6382020
  29. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  30. A DELETION IN YEAST AND ITS BEARING ON THE STRUCTURE OF THE MATING TYPE LOCUS.
    Genetics. 1963 Dec;48:1727-9 PMID: 14105103
  31. The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control.
    Mol Gen Genet. 1976 Jan 16;143(2):119-29 PMID: 765749
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.3831-3837.1986
Published
1986-11-00
Pages
3831-7
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367145
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.11.3831-3837.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2