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PMID: 3025633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mouse immunoglobulin heavy-chain gene enhancer contains sequences that inhibit transcription in vitro in HeLa cell extracts.

Molecular and cellular biology ·Vol. 6 ·No. 11 ·1986-11-00 ·Pages 4117-21

Dougherty JP, Augereau P, Chambon P

Abstract

The effect of the cell-specific mouse immunoglobulin heavy-chain gene (IgH) enhancer on transcription from heterologous promoter elements was studied in vitro with a HeLa whole-cell extract. No stimulation of transcription could be seen under conditions in which an activation was observed with the simian virus 40 enhancer. We found, however, that a specific segment of the IgH enhancer region contains sequences which inhibit transcription in vitro.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Enhancer Elements, Genetic Genes Genes, Regulator HeLa Cells/metabolism Humans Immunoglobulin Heavy Chains/genetics Mice Plasmids Promoter Regions, Genetic Transcription, Genetic
Chemicals
DNA, Recombinant Immunoglobulin Heavy Chains DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dougherty J P
Augereau P
Chambon P
References (20)
20 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.11.4117-4121.1986
Published
1986-11-00
Pages
4117-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367181
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.6.11.4117-4121.1986 PMC Full Text View journal details
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