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PMID: 3027365 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Retrovirus integration and chromatin structure: Moloney murine leukemia proviral integration sites map near DNase I-hypersensitive sites.

Journal of virology ·Vol. 61 ·No. 2 ·1987-02-00 ·Pages 336-43

Rohdewohld H, Weiher H, Reik W, Jaenisch R, Breindl M

Abstract

The chromatin conformation of mouse genome regions containing Moloney murine leukemia proviral intergration sites in two Mov mouse strains and randomly selected integration sites in virus-infected mouse 3T3 fibroblasts was analyzed. All integrations have occurred into chromosomal regions containing several DNase-hypersensitive sites, and invariably the proviral integration sites map within a few hundred base pairs of a DNase-hypersensitive site. The probability that this close association between proviral integration sites and DNase-hypersensitive sites was due to chance was calculated to be extremely low (2 X 10(-4]. Because the proviral integrations analyzed were not selected for an altered phenotype, our results suggest that DNase-hypersensitive regions are preferred targets for retrovirus integration.

MeSH Terms
Animals Cells, Cultured Chromatin/analysis DNA Restriction Enzymes DNA, Recombinant/metabolism Deoxyribonuclease I/metabolism Genes, Viral Mice Moloney murine leukemia virus/genetics Substrate Specificity
Chemicals
Chromatin DNA, Recombinant DNA Restriction Enzymes Deoxyribonuclease I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rohdewohld H
Weiher H
Reik W
Jaenisch R
Breindl M
References (34)
34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.2.336-343.1987
Published
1987-02-00
Pages
336-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253954
Subset
IM
Grants
NICHD NIH HHS · HD-19015 · United States
NCI NIH HHS · P01-CA38497 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.2.336-343.1987 PMC Full Text View journal details
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