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PMID: 6095069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inactivation of p53 gene expression by an insertion of Moloney murine leukemia virus-like DNA sequences.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1402-10

Wolf D, Rotter V

Abstract

Analysis of Abelson murine leukemia virus-transformed L12 cells which lack the p53 cellular encoded tumor antigen revealed alterations in the p53-specific genomic DNA sequences. The active p53 gene, usually contained in a 16-kilobase EcoRI DNA fragment of p53 producer cells, went through major alterations leading to the appearance of a substantially larger 28.0-kilobase p53-specific EcoRI fragment. Detailed restriction enzyme analysis, with genomic probes spanning throughout the whole active p53 gene, indicated that the L12 p53 altered gene contains all the exons and principal introns of the normal p53 16.0-kilobase gene. However, its structure was interrupted by the integration of a novel DNA segment into the noncoding intervening sequences of the first p53 intron. Analysis of the inserted sequences revealed close homology to Moloney murine leukemia virus. This Moloney leukemia murine virus-like particle resides in a 5' to 3' transcriptional orientation, similar to the p53 gene, permitting the transcription of aberrant fused mRNA molecules detected in these cells.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Genes Genes, Viral Leukemia, Experimental/microbiology Mice Mice, Inbred BALB C Neoplasm Proteins/genetics Phosphoproteins/genetics Plasmids Tumor Suppressor Protein p53
Chemicals
DNA, Viral Neoplasm Proteins Phosphoproteins Tumor Suppressor Protein p53 DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wolf D
Rotter V
References (45)
45 references, click to expand
  1. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  2. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  3. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4 PMID: 4559594
  4. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  5. A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus.
    J Exp Med. 1976 Jun 1;143(6):1453-63 PMID: 178822
  6. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  7. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Identification of an Abelson murine leukemia virus-encoded protein present in transformed fibroblast and lymphoid cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2488-92 PMID: 209468
  10. T antigen is bound to a host protein in SV40-transformed cells.
    Nature. 1979 Mar 15;278(5701):261-3 PMID: 218111
  11. Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2420-4 PMID: 221923
  12. In vitro synthesis of a 9 kbp terminally redundant DNA carrying the infectivity of Moloney murine leukemia virus.
    Cell. 1979 Apr;16(4):863-74 PMID: 88264
  13. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells.
    Cell. 1979 May;17(1):43-52 PMID: 222475
  14. Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus-encoded P120 protein at the cell surface.
    J Virol. 1979 Sep;31(3):776-84 PMID: 92572
  15. Host nuclear proteins expressed in simian virus 40-transformed and -infected cells.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):97-101 PMID: 6244576
  16. Relationship among Tau antigens isolated from various lines of simian virus 40-transformed cells.
    J Virol. 1980 Jun;34(3):650-7 PMID: 6247503
  17. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens.
    J Virol. 1980 Jun;34(3):752-63 PMID: 6155477
  18. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  19. Abelson murine leukemia virus-induced tumors elicit antibodies against a host cell protein, P50.
    J Virol. 1980 Nov;36(2):547-55 PMID: 6159484
  20. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  21. An embryo protein induced by SV40 virus transformation of mouse cells.
    Nature. 1980 Dec 25;288(5792):722-4 PMID: 6256642
  22. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  23. p53 transformation-related protein: detection by monoclonal antibody in mouse and human cells.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1695-9 PMID: 6940183
  24. A monoclonal antibody that recognizes B cells and B cell precursors in mice.
    J Exp Med. 1981 Feb 1;153(2):269-79 PMID: 6787164
  25. Increased concentration of an apparently identical cellular protein in cells transformed by either Abelson murine leukemia virus or other transforming agents.
    J Virol. 1981 Apr;38(1):336-46 PMID: 6264107
  26. Detection of a common feature in several human tumor cell lines--a 53,000-dalton protein.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):41-5 PMID: 6264441
  27. p53 transformation-related protein: detection of an associated phosphotransferase activity.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2932-6 PMID: 6265926
  28. SV40-53K antigen: a possible role for 53K in normal cells.
    Virology. 1981 Jul 30;112(2):785-8 PMID: 6266152
  29. Retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus.
    Cell. 1981 Jul;25(1):23-36 PMID: 6268304
  30. SV40 T antigen binds specifically to a cellular 53 K protein in vitro.
    Nature. 1981 Jul 2;292(5818):63-5 PMID: 6268984
  31. Dilute (d) coat colour mutation of DBA/2J mice is associated with the site of integration of an ecotropic MuLV genome.
    Nature. 1981 Oct 1;293(5831):370-4 PMID: 6268990
  32. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  33. Adenovirus E1b-58kd tumor antigen and SV40 large tumor antigen are physically associated with the same 54 kd cellular protein in transformed cells.
    Cell. 1982 Feb;28(2):387-94 PMID: 6277513
  34. Microinjection of monoclonal antibody to protein p53 inhibits serum-induced DNA synthesis in 3T3 cells.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6309-12 PMID: 6292898
  35. Molecular cloning of a cDNA specific for the murine p53 cellular tumor antigen.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):56-9 PMID: 6296874
  36. The presence of p53 transformation-related protein in Ab-MuLV transformed cells is required for their development into lethal tumors in mice.
    Int J Cancer. 1983 Mar 15;31(3):315-20 PMID: 6298128
  37. Intracisternal A-particle genes as movable elements in the mouse genome.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1992-6 PMID: 6300886
  38. p53, a transformation-related cellular-encoded protein, can be used as a biochemical marker for the detection of primary mouse tumor cells.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2613-7 PMID: 6189126
  39. Variation in antigenic determinants of p53 transformation-related protein obtained from various species.
    J Immunol. 1983 Jul;131(1):329-33 PMID: 6190914
  40. Embryonic lethal mutation in mice induced by retrovirus insertion into the alpha 1(I) collagen gene.
    Nature. 1983 Jul 28-Aug 3;304(5924):315-20 PMID: 6308457
  41. Analysis of recombinant DNA clones specific for the murine p53 cellular tumor antigen.
    EMBO J. 1983;2(10):1633-9 PMID: 6315396
  42. A single gene and a pseudogene for the cellular tumour antigen p53.
    Nature. 1983 Dec 8-14;306(5943):594-7 PMID: 6646235
  43. Chromosomal assignment of the murine gene encoding the transformation-related protein p53.
    Mol Cell Biol. 1984 Feb;4(2):383-5 PMID: 6366521
  44. Abelson murine leukemia virus-transformed cells that lack p53 protein synthesis express aberrant p53 mRNA species.
    Mol Cell Biol. 1984 Mar;4(3):552-8 PMID: 6144042
  45. Post-translational regulation of the 54K cellular tumor antigen in normal and transformed cells.
    Mol Cell Biol. 1981 Feb;1(2):101-10 PMID: 6100960
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1402-1410.1984
Published
1984-07-00
Pages
1402-10
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368923
Subset
IM
Databases
GENBANK
K02704, K02705
External Links
PubMed source DOI: 10.1128/mcb.4.7.1402-1410.1984 PMC Full Text View journal details
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