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PMID: 3029037 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase.

Journal of bacteriology ·Vol. 169 ·No. 3 ·1987-03-00 ·Pages 949-54

Conway T, Osman YA, Konnan JI, Hoffmann EM, Ingram LO

Abstract

DNA sequence analysis showed that pyruvate decarboxylase (one of the most abundant proteins in Zymomonas mobilis) contains 559 amino acids. The promoter for the gene encoding pyruvate decarboxylase was not recognized by Escherichia coli, although the cloned gene was expressed at relatively high levels under the control of alternative promoters. The promoter region did not contain sequences which could be identified as being homologous to the generalized promoter structure for E. coli. Hydropathy plots for the amino acid sequence indicated that pyruvate decarboxylase contains a large number of hydrophobic domains which may contribute to the thermal stability of this enzyme.

MeSH Terms
Amino Acid Sequence Base Sequence Carboxy-Lyases/genetics Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Gram-Negative Bacteria/enzymology,genetics Promoter Regions, Genetic Pyruvate Decarboxylase/genetics,isolation & purification
Chemicals
DNA Restriction Enzymes Carboxy-Lyases Pyruvate Decarboxylase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Conway T
Osman Y A
Konnan J I
Hoffmann E M
Ingram L O
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.3.949-954.1987
Published
1987-03-00
Pages
949-54
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211885
Subset
IM
Databases
GENBANK
M15393
External Links
PubMed source DOI: 10.1128/jb.169.3.949-954.1987 PMC Full Text View journal details
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