Home LiteratureArticle Details
PMID: 3029695 Published · ppublish English Journal Article

Expression and identification of immunity determinants on linear DNA killer plasmids pGKL1 and pGKL2 in Kluyveromyces lactis.

Nucleic acids research ·Vol. 15 ·No. 3 ·1987-02-11 ·Pages 1031-46

Tokunaga M, Wada N, Hishinuma F

Abstract

The linear dsDNA plasmids, pGKL1 (8.9 kb) and pGKL2 (13.4 kb) discovered in Kluyveromyces lactis, confer killer and immunity characteristics upon various yeast strains. We have devised an immunity assay and have been able to show the expression of an immunity phenotype in the K. lactis transformants harbouring conventional circular plasmids which contain DNA fragments of pGKL1. Using this expression system, the immunity determinant on pGKL1 was identified as ORF5. In addition, the presence of pGKL2 was proved to be essential for the expression of the immunity phenotype. This is the first demonstration of this new pGKL2 function, as distinct from its known functions for the replication and maintenance of pGKL1 in yeast cells.

MeSH Terms
DNA Restriction Enzymes DNA, Fungal/genetics Fungal Proteins/genetics Killer Factors, Yeast Kluyveromyces/genetics,immunology Mycotoxins/genetics Plasmids RNA, Double-Stranded/genetics Saccharomycetales/genetics
Chemicals
DNA, Fungal Fungal Proteins Killer Factors, Yeast Mycotoxins RNA, Double-Stranded DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tokunaga M
Wada N
Hishinuma F
References (24)
24 references, click to expand
  1. Double-stranded ribonucleic acid killer systems in yeasts.
    Microbiol Rev. 1984 Jun;48(2):125-56 PMID: 6377033
  2. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903.
    Gene. 1984 Apr;28(1):73-81 PMID: 6329916
  3. The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins.
    Nucleic Acids Res. 1984 Jul 25;12(14):5685-92 PMID: 6379603
  4. Nucleotide sequence and transcription analysis of a linear DNA plasmid associated with the killer character of the yeast Kluyveromyces lactis.
    Nucleic Acids Res. 1984 Aug 10;12(15):6011-30 PMID: 6473099
  5. Cloning and nucleotide sequences of the linear DNA killer plasmids from yeast.
    Nucleic Acids Res. 1984 Oct 11;12(19):7581-97 PMID: 6387625
  6. Identification of prolipoprotein signal peptidase and genomic organization of the lsp gene in Escherichia coli.
    J Biol Chem. 1985 May 10;260(9):5610-5 PMID: 2580835
  7. Immobilization and purification of enzymes with staphylococcal protein A gene fusion vectors.
    EMBO J. 1985 Apr;4(4):1075-80 PMID: 2990908
  8. Transfer of DNA killer plasmids from Kluyveromyces lactis to Kluyveromyces fragilis and Candida pseudotropicalis.
    J Bacteriol. 1985 Dec;164(3):1373-5 PMID: 4066615
  9. Expression of a cDNA derived from the yeast killer preprotoxin gene: implications for processing and immunity.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1675-9 PMID: 3513184
  10. Yeast killer toxin: site-directed mutations implicate the precursor protein as the immunity component.
    Cell. 1986 Jul 4;46(1):105-13 PMID: 3521889
  11. The killer toxin of Kluyveromyces lactis: characterization of the toxin subunits and identification of the genes which encode them.
    EMBO J. 1986 Aug;5(8):1995-2002 PMID: 3758030
  12. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  13. Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character.
    J Bacteriol. 1981 Jan;145(1):382-90 PMID: 6257636
  14. Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion.
    J Bacteriol. 1981 Jul;147(1):155-60 PMID: 7016841
  15. Nucleotide sequence of the kanamycin resistance transposon Tn903.
    J Mol Biol. 1981 Apr 5;147(2):217-26 PMID: 6270337
  16. Curing of the killer deoxyribonucleic acid plasmids of Kluyveromyces lactis.
    J Bacteriol. 1981 Dec;148(3):988-90 PMID: 7309684
  17. Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.
    J Bacteriol. 1982 Jul;151(1):462-4 PMID: 7045080
  18. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  19. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  20. Kluyveromyces lactis killer toxin inhibits adenylate cyclase of sensitive yeast cells.
    Nature. 1983 Aug 4-10;304(5925):464-6 PMID: 6192345
  21. Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae.
    Gene. 1983 Sep;24(1):1-14 PMID: 6313478
  22. A distinct signal peptidase for prolipoprotein in Escherichia coli.
    J Cell Biochem. 1984;24(2):113-20 PMID: 6373799
  23. Characterization of a novel killer toxin encoded by a double-stranded linear DNA plasmid of Kluyveromyces lactis.
    Eur J Biochem. 1984 Jun 1;141(2):241-5 PMID: 6734597
  24. The expression of cDNA clones of yeast M1 double-stranded RNA in yeast confers both killer and immunity phenotypes.
    EMBO J. 1984 Jun;3(6):1383-7 PMID: 6086322
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.3.1031
Published
1987-02-11
Pages
1031-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340506
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.3.1031 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2