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PMID: 3031452 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of specific topoisomerase I interactions within the transcribed region of active heat shock genes by using the inhibitor camptothecin.

Molecular and cellular biology ·Vol. 7 ·No. 1 ·1987-01-00 ·Pages 141-8

Gilmour DS, Elgin SC

Abstract

Camptothecin stabilizes the topoisomerase I-DNA covalent intermediate that forms during the relaxation of torsionally strained DNA. By mapping the position of the resultant DNA nicks, we analyzed the distribution of the covalent intermediates formed on heat shock genes in cultured Drosophila melanogaster cells. Topoisomerase I was found to interact with the transcriptionally active genes hsp22, hsp23, hsp26, and hsp28 after heat shock but not with the inactive genes before heat shock. The interaction occurred predominantly within the transcribed region, with specific sites occurring on both the transcribed and nontranscribed strands of the DNA. Little interaction was seen with nontranscribed flanking sequences. Camptothecin only partially inhibited transcription of the hsp28 gene during heat shock, causing a reduced level of transcripts which were nonetheless full length. Topoisomerase I also interacted with the DNA throughout the transcriptionally active hsp83 gene, including an intron, in both heat-shocked and non-heat-shocked cells. The results point to a dynamic set of interactions at the active locus.

MeSH Terms
Animals Camptothecin/pharmacology Cells, Cultured DNA Topoisomerases, Type I/metabolism Drosophila melanogaster/genetics Genes/drug effects Heat-Shock Proteins/genetics Substrate Specificity Transcription, Genetic/drug effects
Chemicals
Heat-Shock Proteins DNA Topoisomerases, Type I Camptothecin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilmour D S
Elgin S C
References (46)
46 references, click to expand
  1. Novobiocin blocks the Drosophila heat shock response.
    J Mol Biol. 1985 May 5;183(1):13-29 PMID: 2989538
  2. In situ localization of DNA topoisomerase II, a major polypeptide component of the Drosophila nuclear matrix fraction.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4142-6 PMID: 2987966
  3. Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I.
    J Biol Chem. 1985 Nov 25;260(27):14873-8 PMID: 2997227
  4. Cloning of yeast TOP1, the gene encoding DNA topoisomerase I, and construction of mutants defective in both DNA topoisomerase I and DNA topoisomerase II.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7178-82 PMID: 2997777
  5. Association of crossover points with topoisomerase I cleavage sites: a model for nonhomologous recombination.
    Science. 1985 Nov 22;230(4728):954-8 PMID: 2997924
  6. Topoisomerase I interacts with transcribed regions in Drosophila cells.
    Cell. 1986 Feb 14;44(3):401-7 PMID: 3002635
  7. Gyration is required for 5S RNA transcription from a chromatin template.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1305-9 PMID: 3006044
  8. Both DNA topoisomerases I and II relax 2 micron plasmid DNA in living yeast cells.
    Cell. 1986 Apr 11;45(1):65-70 PMID: 3006927
  9. Multiple forms and cellular localization of Drosophila DNA topoisomerase II.
    J Biol Chem. 1986 Jun 15;261(17):8063-9 PMID: 3011806
  10. In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster.
    Mol Cell Biol. 1985 Aug;5(8):2009-18 PMID: 3018544
  11. Nucleosomal instability and induction of new upstream protein-DNA associations accompany activation of four small heat shock protein genes in Drosophila melanogaster.
    Mol Cell Biol. 1986 Mar;6(3):779-91 PMID: 3022129
  12. In vivo localization of DNA topoisomerase II cleavage sites on Drosophila heat shock chromatin.
    Mol Cell Biol. 1986 Apr;6(4):985-92 PMID: 3023886
  13. An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6 PMID: 4333036
  14. An omega protein from Drosophila melanogaster.
    Biochemistry. 1974 Oct 8;13(21):4299-303 PMID: 4212937
  15. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  16. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  17. The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
    Cell. 1979 Apr;16(4):807-14 PMID: 455450
  18. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  19. Nicking-closing enzyme assembles nucleosome-like structures in vitro.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3779-83 PMID: 226980
  20. Non-random cleavage of SV40 DNA in the compact minichromosome and free in solution by micrococcal nuclease.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):532-9 PMID: 6243943
  21. The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
    Nature. 1980 Aug 28;286(5776):854-60 PMID: 6774262
  22. Four Drosophila heat shock genes at 67B: characterization of recombinant plasmids.
    Nucleic Acids Res. 1980 Oct 10;8(19):4441-57 PMID: 6253919
  23. Four heat shock proteins of Drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67B.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5390-3 PMID: 6254079
  24. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  25. Two closely linked transcription units within the 63B heat shock puff locus of D. melanogaster display strikingly different regulation.
    Nucleic Acids Res. 1981 Oct 10;9(19):5075-92 PMID: 6118854
  26. Developmentally regulated transcription from Drosophila melanogaster chromosomal site 67B.
    Dev Biol. 1982 Jan;89(1):196-210 PMID: 6274724
  27. The ovalbumin gene is associated with the nuclear matrix of chicken oviduct cells.
    Cell. 1982 Jan;28(1):99-106 PMID: 7066988
  28. Drosophila topoisomerase I: isolation, purification and characterization.
    Nucleic Acids Res. 1982 Nov 11;10(21):6945-55 PMID: 6294622
  29. Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
    Nature. 1983 Mar 3;302(5903):38-43 PMID: 6828158
  30. Localization of the hsp83 transcript within a 3292 nucleotide sequence from the 63B heat shock locus of D. melanogaster.
    Nucleic Acids Res. 1983 Oct 25;11(20):7011-30 PMID: 6314271
  31. A site and strand specific nuclease activity with analogies to topoisomerase I frames the rRNA gene of Tetrahymena.
    Nucleic Acids Res. 1983 Nov 25;11(22):7661-78 PMID: 6316268
  32. DNA supercoiling and gene expression.
    Nature. 1984 Feb 23-29;307(5953):686-7 PMID: 6321994
  33. DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2616-20 PMID: 6326134
  34. Chromatin assembly in Xenopus oocytes: in vivo studies.
    Cell. 1984 May;37(1):21-32 PMID: 6202419
  35. Elastic torsional strain in DNA within a fraction of SV40 minichromosomes: relation to transcriptionally active chromatin.
    EMBO J. 1982;1(11):1353-8 PMID: 6327265
  36. A cytological approach to the ordering of events in gene activation using the Sgs-4 locus of Drosophila melanogaster.
    J Cell Biol. 1984 Jul;99(1 Pt 1):233-8 PMID: 6330126
  37. Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
    EMBO J. 1984 Aug;3(8):1737-44 PMID: 6090122
  38. Nonintercalative antitumor drugs interfere with the breakage-reunion reaction of mammalian DNA topoisomerase II.
    J Biol Chem. 1984 Nov 10;259(21):13560-6 PMID: 6092381
  39. High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Sep;4(9):1853-63 PMID: 6436689
  40. Drosophila DNA topoisomerase I is associated with transcriptionally active regions of the genome.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6958-62 PMID: 6095263
  41. Torsional stress promotes the DNAase I sensitivity of active genes.
    Cell. 1984 Dec;39(3 Pt 2):469-78 PMID: 6096006
  42. A high affinity topoisomerase I binding sequence is clustered at DNAase I hypersensitive sites in Tetrahymena R-chromatin.
    Cell. 1985 Jun;41(2):541-51 PMID: 2985282
  43. DNA topoisomerase II is required at the time of mitosis in yeast.
    Cell. 1985 Jun;41(2):553-63 PMID: 2985283
  44. Topoisomerase II is a structural component of mitotic chromosome scaffolds.
    J Cell Biol. 1985 May;100(5):1706-15 PMID: 2985625
  45. DNA topoisomerases: enzymes that control DNA conformation.
    Curr Top Microbiol Immunol. 1985;114:19-102 PMID: 2986908
  46. Transcription occurs at a nucleoskeleton.
    EMBO J. 1985 Apr;4(4):919-25 PMID: 2990913
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.1.141-148.1987
Published
1987-01-00
Pages
141-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365050
Subset
IM
Grants
NIGMS NIH HHS · GM10798-01 · United States
NIGMS NIH HHS · GM30273 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.1.141-148.1987 PMC Full Text View journal details
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