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PMID: 3031591 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Activation of the adenovirus 2 protein IX promoter by DNA replication in a transient expression assay.

Nucleic acids research ·Vol. 15 ·No. 5 ·1987-03-11 ·Pages 2235-50

Venkatesh LK, Chinnadurai G

Abstract

We have studied the effect of template replication on transcriptional activation of the promoters for the adenoviral protein IX and E1a genes using a short-term transient expression assay. DNA replication mediated modulation of the transcriptional activity of these promoters was monitored using plasmids with limited replication capability conferred by the SV40 minimal origin of DNA replication in the monkey kidney cell line, COS M6. Our results indicate a substantial increase in protein IX promoter directed transcription in a dosage-independent manner as a consequence of the DNA replication process. In contrast, the transcriptional activity of the adenovirus 2 E1a promoter was not significantly altered. In addition to this DNA replication-mediated effect, transcription from the protein IX promoter was positively regulated by the adenovirus E1a 13S and E4 gene products but was repressed by the E1a 12S gene product to a limited extent. The evidence suggests that the effect of template replication on the transcriptional activity of plasmid-borne protein IX and E1a gene promoters in this transient expression system closely mimics the situation on the viral chromosome.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Animals Cell Line DNA Replication DNA Restriction Enzymes Genes Genes, Viral HeLa Cells Humans Oncogene Proteins, Viral/genetics Promoter Regions, Genetic RNA, Messenger/genetics Templates, Genetic Transcription, Genetic Viral Proteins/genetics
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral RNA, Messenger Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Venkatesh L K
Chinnadurai G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.5.2235
Published
1987-03-11
Pages
2235-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340630
Subset
IM
Grants
NCI NIH HHS · CA31719 · United States
External Links
PubMed source DOI: 10.1093/nar/15.5.2235 PMC Full Text View journal details
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