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PMID: 3032897 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI plasmids P307 and F, and its relation to the RepA replicon of IncFII plasmids.

Journal of bacteriology ·Vol. 169 ·No. 5 ·1987-05-00 ·Pages 1836-46

Saadi S, Maas WK, Hill DF, Bergquist PL

Abstract

RepFIC is a basic replicon of IncFI plasmid P307 which is located within a 3.09-kilobase SmaI fragment. The nucleotide sequence of this region has been determined and shown to be homologous with the RepFIIA replicon of IncFII plasmids. The two replicons share three homologous regions, HRI, HRII, and HRIII, which are flanked by two nonhomologous regions, NHRI and NHRII. A comparison of coding regions reveals that the two replicons have several features in common. RepFIC, like RepFIIA, codes for a repA2 protein with its amino-terminal codons in HRI and its carboxy-terminal codons in NHRI. Although the codons for the repA1 proteins are located in NHRII, the DNA region containing a putative promoter, ribosomal binding site, and initiation codons is located in HRII. This region also codes for an inc RNA. There are nine base-pair differences between the inc RNA of RepFIIA and that of RepFIC, and as a result, RepFIC and RepFIIA replicons are compatible. An EcoRI fragment from the F plasmid which shows homology with RepFIC of P307 has also been sequenced. This fragment contains only a portion of RepFIC, including the genes for the putative repA2 protein and inc RNA. The region coding for a putative repA1 protein is interrupted by the transposon Tn1000 and shows no homology with the repA1 region of RepFIIA and RepFIC of P307. Our comparative and structural analyses suggest that RepFIC and RepFIIA, although different, have a similar replication mechanism and thus can be assigned to the same replicon family, which we designate the RepFIIA family.

MeSH Terms
Base Sequence DNA Transposable Elements DNA, Bacterial/genetics Gene Expression Regulation Plasmids Replicon Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Saadi S
Maas W K
Hill D F
Bergquist P L
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.5.1836-1846.1987
Published
1987-05-00
Pages
1836-46
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212033
Subset
IM
Grants
NIAID NIH HHS · AI07180 · United States
NIGMS NIH HHS · GM06048 · United States
NIGMS NIH HHS · GM15129 · United States
Databases
GENBANK
M16167, M16168, M22298
External Links
PubMed source DOI: 10.1128/jb.169.5.1836-1846.1987 PMC Full Text View journal details
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