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PMID: 3033440 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The yeast nuclear gene CBS1 is required for translation of mitochondrial mRNAs bearing the cob 5' untranslated leader.

Molecular & general genetics : MGG ·Vol. 206 ·No. 1 ·1987-01-00 ·Pages 45-50

Rödel G, Fox TD

Abstract

Mitochondrial translation of the cob mRNA to yield apocytochrome b is specifically dependent on the nuclear gene CBS1, while mitochondrial translation of the oxi2 mRNA to yield cytochrome oxidase subunit III (cox III) is specifically dependent on the nuclear gene PET494. Chimeric oxi2 mRNAs bearing the 5' leaders of other mitochondrial mRNAs, transcribed from rho- mitochondrial DNAs termed MSU494, are translated in pet494 mutants. In this study, we examined translation of coxIII from MSU494-encoded chimeric mRNAs in zygotes of defined nuclear and mitochondrial genotype. CoxIII was translated from a chimeric mRNA bearing the cob leader only when the zygotes contained a wild-type CBS1 gene. CoxIII translation from an mRNA bearing the 5' leader of the mitochondrial gene aap1 was not dependent on CBS1 activity. We conclude that the product of the nuclear gene CBS1, or something under its control, acts in the mitochondrion on the cob mRNA 5' leader to activate translation of down-stream coding sequences.

MeSH Terms
Cell Nucleus/metabolism Electron Transport Complex IV/genetics Genes Genes, Fungal Mitochondria/enzymology Protein Biosynthesis Protein Sorting Signals/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Protein Sorting Signals RNA, Messenger Electron Transport Complex IV
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rödel G
Fox T D
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32 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00326534
Published
1987-01-00
Pages
45-50
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM29362 · United States
NICHD NIH HHS · HDO0515 · United States
External Links
PubMed source DOI: 10.1007/BF00326534
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