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PMID: 3036779 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Site-specific methylases induce the SOS DNA repair response in Escherichia coli.

Journal of bacteriology ·Vol. 169 ·No. 7 ·1987-07-00 ·Pages 3243-50

Heitman J, Model P

Abstract

Expression of the site-specific adenine methylase HhaII (GmeANTC, where me is methyl) or PstI (CTGCmeAG) induced the SOS DNA repair response in Escherichia coli. In contrast, expression of methylases indigenous to E. coli either did not induce SOS (EcoRI [GAmeATTC] or induced SOS to a lesser extent (dam [GmeATC]). Recognition of adenine-methylated DNA required the product of a previously undescribed gene, which we named mrr (methylated adenine recognition and restriction). We suggest that mrr encodes an endonuclease that cleaves DNA containing N6-methyladenine and that DNA double-strand breaks induce the SOS response. Cytosine methylases foreign to E. coli (MspI [meCCGG], HaeIII [GGmeCC], BamHI [GGATmeCC], HhaI [GmeCGC], BsuRI [GGmeCC], and M.Spr) also induced SOS, whereas one indigenous to E. coli (EcoRII [CmeCA/TGG]) did not. SOS induction by cytosine methylation required the rglB locus, which encodes an endonuclease that cleaves DNA containing 5-hydroxymethyl- or 5-methylcytosine (E. A. Raleigh and G. Wilson, Proc. Natl. Acad. Sci. USA 83:9070-9074, 1986).

MeSH Terms
5-Methylcytosine Adenine/analogs & derivatives,physiology Bacteriophage lambda/genetics Cloning, Molecular Cytosine/analogs & derivatives,physiology DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Damage DNA Repair DNA Transposable Elements Escherichia coli/genetics Lysogeny Methyltransferases/genetics,metabolism Mutation Promoter Regions, Genetic SOS Response, Genetics Site-Specific DNA-Methyltransferase (Adenine-Specific)
Chemicals
DNA Transposable Elements 5-Methylcytosine Cytosine DNA modification methylase HinfI Methyltransferases DNA (Cytosine-5-)-Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) Adenine 6-methyladenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heitman J
Model P
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.7.3243-3250.1987
Published
1987-07-00
Pages
3243-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212376
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.7.3243-3250.1987 PMC Full Text View journal details
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