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PMID: 6096817 Published · ppublish English Comparative Study Journal Article

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 9039-49

Pósfai G, Baldauf F, Erdei S, Pósfai J, Venetianer P, Kiss A

Abstract

The nucleotide sequence of the gene coding for the 5'-GGCC and 5'-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5'-GGCC-specific BspRI modification methylase.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacteriophages/enzymology,genetics Base Sequence DNA (Cytosine-5-)-Methyltransferases/genetics DNA Restriction Enzymes Endonucleases Escherichia coli/enzymology,genetics Genes Genes, Bacterial Methyltransferases/genetics Protein Biosynthesis Single-Strand Specific DNA and RNA Endonucleases Species Specificity
Chemicals
Methyltransferases DNA (Cytosine-5-)-Methyltransferases Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pósfai G
Baldauf F
Erdei S
Pósfai J
Venetianer P
Kiss A
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.23.9039
Published
1984-12-11
Pages
9039-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320436
Subset
IM
Databases
GENBANK
K02124, X01670
External Links
PubMed source DOI: 10.1093/nar/12.23.9039 PMC Full Text View journal details
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