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PMID: 3037312 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Adeno-associated virus gene expression inhibits cellular transformation by heterologous genes.

Molecular and cellular biology ·Vol. 7 ·No. 4 ·1987-04-00 ·Pages 1320-5

Labow MA, Graf LH, Berns KI

Abstract

In this paper we report that adeno-associated virus (AAV) genomes inhibit stable transformation by several dominant selectable marker genes upon cotransfection into mouse tissue culture cells. Cotransfection of AAV genomes also inhibited the expression of pSV2cat in transient assays. In both cases, the inhibitory effect was independent of AAV DNA replication but required the AAV p5 and p19 genes, which encode proteins required for AAV DNA replication and regulation of AAV gene expression. Finally, addition of a cloned E4 gene in the transfection experiments partially blocked the AAV-mediated inhibitory activities.

MeSH Terms
Acetyltransferases/genetics Adenovirus Early Proteins Animals Cell Line Cell Transformation, Neoplastic Chloramphenicol O-Acetyltransferase DNA Replication Dependovirus/genetics Genes Genes, Viral Melanoma, Experimental Mice Mutation Oncogene Proteins, Viral/genetics Plasmids Transfection
Chemicals
Adenovirus Early Proteins Oncogene Proteins, Viral Acetyltransferases Chloramphenicol O-Acetyltransferase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Labow M A
Graf L H
Berns K I
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41 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.4.1320-1325.1987
Published
1987-04-00
Pages
1320-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365216
Subset
IM
Grants
NIAID NIH HHS · AI 22251 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.4.1320-1325.1987 PMC Full Text View journal details
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