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PMID: 3037326 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Functional simian virus 40 T antigen is expressed in hybrid cells having finite proliferative potential.

Molecular and cellular biology ·Vol. 7 ·No. 4 ·1987-04-00 ·Pages 1541-4

Pereira-Smith OM, Smith JR

Abstract

Simian virus 40-transformed human cells fused with other independently derived simian virus 40-transformed cells and tumor-derived cells containing activated H-ras and N-ras oncogenes yielded hybrids capable of indefinite division. Fusions with various other immortal cells yielded hybrids that had limited division potential. T antigen expressed in limited-division hybrids was functional for the induction of cellular DNA synthesis.

MeSH Terms
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics Cell Division Cell Line Cell Transformation, Neoplastic Cell Transformation, Viral DNA Replication Humans Hybrid Cells/cytology,enzymology Oncogene Proteins, Viral/genetics Oncogenes Protein Kinases/genetics Simian virus 40/genetics
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Protein Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pereira-Smith O M
Smith J R
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28 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.4.1541-1544.1987
Published
1987-04-00
Pages
1541-4
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365243
Subset
IM
Grants
NIA NIH HHS · AG04749 · United States
NIA NIH HHS · AG05333 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.4.1541-1544.1987 PMC Full Text View journal details
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