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PMID: 3037492 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The SV40 termination region exhibits an altered helical DNA conformation.

Nucleic acids research ·Vol. 15 ·No. 13 ·1987-07-10 ·Pages 5433-42

Poljak LG, Gralla JD

Abstract

The DNA structure of a fragment containing the SV40 termination sequences was examined using gel mobility assays. The region is shown to contain a DNA bend as evidenced by an abnormal mobility that is progressively accentuated as the temperature is lowered. This represents the strongest example of DNA bending among the collection of SV40 fragments studied. The same fragment was shown previously to uniquely support hyper-stable nucleosome formation in vitro, suggesting a possible relationship between DNA bending and nucleosome stability.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Viral/genetics Genes, Viral Kinetics Nucleic Acid Conformation Simian virus 40/genetics Thermodynamics
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Poljak L G
Gralla J D
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25 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.13.5433
Published
1987-07-10
Pages
5433-42
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305970
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.13.5433 PMC Full Text View journal details
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