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PMID: 3037539 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional analysis of a retroviral host-range mutant: altered long terminal repeat sequences allow expression in embryonal carcinoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 15 ·1987-08-00 ·Pages 5232-6

Hilberg F, Stocking C, Ostertag W, Grez M

Abstract

A retroviral host-range neomycin-resistant myeloproliferative sarcoma virus mutant, which is expressed in the embryonal carcinoma cell lines F9 and PCC4aza1R, was molecularly cloned and analyzed. This mutant virus, PCMV, differs from myeloproliferative sarcoma virus by two major deletions, one of which spans exactly a 75-base-pair repeat of the long terminal repeat. Functional analysis of recombinant viruses shows that the host-range expansion of PCMV is a property of nucleotide changes within the U3 region of the long terminal repeat. Furthermore, expression assays of chimeric long terminal repeats show that the enhancer region of PCMV joined to the promoter region of Moloney murine leukemia virus is sufficient to direct the synthesis of chloramphenicol acetyltransferase in F9 and PCC4 cells.

MeSH Terms
Animals Base Sequence Cell Line Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes/metabolism DNA, Viral/analysis Drug Resistance, Microbial/genetics Gene Expression Regulation Mutation Neomycin/pharmacology Repetitive Sequences, Nucleic Acid Retroviridae/genetics Teratoma/genetics Transcription, Genetic
Chemicals
DNA, Viral DNA Restriction Enzymes Neomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hilberg F
Stocking C
Ostertag W
Grez M
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39 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.15.5232
Published
1987-08-00
Pages
5232-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298829
Subset
IM
Databases
GENBANK
M17246
External Links
PubMed source DOI: 10.1073/pnas.84.15.5232 PMC Full Text View journal details
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