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PMID: 3039353 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Unusual sequence element found at the end of an amplicon.

Molecular and cellular biology ·Vol. 7 ·No. 7 ·1987-07-00 ·Pages 2636-40

Baran N, Lapidot A, Manor H

Abstract

In a polyomavirus-transformed rat cell line, designated LPT, the polyomavirus DNA is integrated into a single chromosomal site. Treatment of LPT cells with carcinogens induces amplification of the integrated virus DNA and flanking cellular sequences. We show that the amplification is arrested within a specific cell DNA segment that maps 1.3 to 1.85 kilobases beyond one virus-cell DNA junction, defined as the left junction. We also present the sequence of an 897-base-pair fragment spanning the arrest site. This fragment contains an unusual sequence element, which consists of two contiguous components, a potential cruciform with stems of 6 base pairs and a d(G-A)27 X d(T-C)27 tract, and maps 1,497 to 1,564 nucleotides beyond the left junction. The possibility that this unusual sequence plays a role in the arrest of the amplification process is discussed.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral Chromosome Mapping DNA, Viral/genetics Gene Amplification Genes, Viral Polyomavirus/genetics Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baran N
Lapidot A
Manor H
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26 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.7.2636-2640.1987
Published
1987-07-00
Pages
2636-40
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365404
Subset
IM
Databases
GENBANK
M16716
External Links
PubMed source DOI: 10.1128/mcb.7.7.2636-2640.1987 PMC Full Text View journal details
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